Wang Yifu, Li Jinjin, Ding Linlin, Gao Xiaolan, Chi Zhilan, Ma Shanshan, He Leiliang, Xiong Yamin
School of Life Sciences, Zhengzhou University, Zhengzhou 450001, China; College of Public Health, Zhengzhou University, Zhengzhou 450001, China.
School of Life Sciences, Zhengzhou University, Zhengzhou 450001, China.
Int J Biol Macromol. 2025 Jun 16;319(Pt 2):145326. doi: 10.1016/j.ijbiomac.2025.145326.
Bacterial infection reduces quality of wound healing and even cause severe complications, but most current dressings adopt single antibacterial mode showed limited effectiveness. Herein, a plant extract quercetin (Que) composite chitosan/hyaluronic acid hydrogel (named CBCQ) with near-infrared (NIR) triggered controllable NO releasing was developed for efficient photothermal therapy/nitric oxide (PTT/NO) synergistic antibacterial and wound healing. Specifically, CBCQ hydrogel with chemical/physical crosslinked network was fabricated via host-guest interaction, metal coordination and Schiff base reaction. It exhibited porous structure, excellent injectability, self-healing, adhesion, antioxidant, and biocompatible properties suitable for wound dressings. Notably, superior PTT effect generated from Que-Fe has been demonstrated, which served as a photothermal switch for the controllable NO release, thereby enable to utilize the physiological functions of NO at different concentrations, improving antibacterial efficiency by integrating PTT and NO benefits, and further allowing sustained antibacterial through Que when NIR was off. Importantly, CBCQ hydrogel achieved better healing of skin incision than commercial 3M medical glue and Film, and significantly improved the healing rate and quality of Methicillin-resistant Staphylococcus aureus (MRSA) infected wound by efficient and sustained antibacterial activity, inhibiting inflammation, and promoting collagen deposition and angiogenesis, highlighting its promising application in skin incisions and infected full-thickness skin wounds.
细菌感染会降低伤口愈合质量，甚至引发严重并发症，但目前大多数敷料采用单一抗菌模式，效果有限。在此，我们开发了一种具有近红外（NIR）触发可控一氧化氮释放功能的植物提取物槲皮素（Que）复合壳聚糖/透明质酸水凝胶（命名为CBCQ），用于高效光热疗法/一氧化氮（PTT/NO）协同抗菌和伤口愈合。具体而言，通过主客体相互作用、金属配位和席夫碱反应制备了具有化学/物理交联网络的CBCQ水凝胶。它具有多孔结构、优异的可注射性、自愈性、粘附性、抗氧化性和生物相容性，适合作为伤口敷料。值得注意的是，已证明Que-Fe产生的优异光热效应，其作为可控一氧化氮释放的光热开关，从而能够利用不同浓度一氧化氮的生理功能，通过整合PTT和一氧化氮的优势提高抗菌效率，并在近红外关闭时通过Que实现持续抗菌。重要的是，CBCQ水凝胶在皮肤切口愈合方面比市售3M医用胶水和薄膜表现更好，通过高效且持续的抗菌活性、抑制炎症以及促进胶原蛋白沉积和血管生成，显著提高了耐甲氧西林金黄色葡萄球菌（MRSA）感染伤口的愈合率和质量，突出了其在皮肤切口和感染的全层皮肤伤口中的应用前景。
