Liu Mi, Zhao Weidan, Li Denghao, Cui Dingyu, Liu Jiali, Chen Qingao, Zhou Yan, Jiang Li, Zhou Huaibin
College of Optical and Electronic Technology, China Jiliang University, Hangzhou, Zhejiang 310018, China.
Key Laboratory of Laboratory Medicine, Ministry of Education, Zhejiang Provincial Key Laboratory of Medical Genetics, College of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China.
Langmuir. 2025 Jul 8;41(26):17104-17116. doi: 10.1021/acs.langmuir.5c01761. Epub 2025 Jun 26.
Infected wound healing remains a significant clinical challenge due to bacterial resistance and excessive inflammation. Herein, a multifunctional hydrogel dressing (NbC-Arg/Gel) is developed that integrates two-dimensional NbC nanosheets (photothermal agent) and arginine [nitric oxide (NO) donor] into a calcium ion cross-linked sodium alginate matrix. The hydrogel has a relatively high swelling capacity and good ability to scavenge reactive oxygen species (ROS), which are beneficial for absorbing wound exudate and reducing the inflammatory response of wounds. Under irradiation with a low-power NIR-II laser (1064 nm, 1 W cm), NbC-Arg/Gel achieves rapid photothermal heating (48.6 °C in 1 min), while thermally enhances arginine decomposition-triggered on-demand NO release. This synergistic mechanism provides superior antimicrobial efficacy against (97.65%) and methicillin-resistant (90.05%). In vivo studies on MRSA-infected murine wounds demonstrate that the residual area on day 10 was 2.9%, which accelerated healing compared to that of the control group (17.8%), accompanied by reduced inflammation and enhanced angiogenesis. The excellent biocompatibility of hydrogel is validated by low hemolysis (<5%) and promoted fibroblast proliferation (125% cell viability). Combining with photothermal, NO-mediated antibacterial action, and ROS scavenging to alleviate oxidative stress, this antibiotic-free hydrogel dressing has great potential for clinical application for the treatment of refractory-infected wounds.
由于细菌耐药性和过度炎症反应，感染伤口的愈合仍然是一个重大的临床挑战。在此，开发了一种多功能水凝胶敷料（NbC-Arg/Gel），它将二维NbC纳米片（光热剂）和精氨酸[一氧化氮（NO）供体]整合到钙离子交联的海藻酸钠基质中。该水凝胶具有较高的溶胀能力和良好的清除活性氧（ROS）的能力，有利于吸收伤口渗出液并减轻伤口的炎症反应。在低功率近红外二区激光（1064 nm，1 W/cm）照射下，NbC-Arg/Gel实现快速光热加热（1分钟内达到48.6℃），同时热增强精氨酸分解触发的按需NO释放。这种协同机制对金黄色葡萄球菌（97.65%）和耐甲氧西林金黄色葡萄球菌（90.05%）具有优异的抗菌效果。对耐甲氧西林金黄色葡萄球菌感染的小鼠伤口进行的体内研究表明，第10天的残留面积为2.9%，与对照组（17.8%）相比，愈合加速，同时炎症减轻，血管生成增强。水凝胶的优异生物相容性通过低溶血率（<5%）和促进成纤维细胞增殖（细胞活力125%）得到验证。结合光热、NO介导的抗菌作用和ROS清除以减轻氧化应激，这种无抗生素的水凝胶敷料在治疗难治性感染伤口的临床应用中具有巨大潜力。
