Doumas B T, Kwok-Cheung P P, Perry B W, Jendrzejczak B, McComb R B, Schaffer R, Hause L L
Clin Chem. 1985 Nov;31(11):1779-89.
This candidate Reference Method for measuring total bilirubin in serum is based on the Jendrassik-Gróf principle (Clin Chem 29: 297-301, 1983). Standard Reference Material no. 916 bilirubin (National Bureau of Standards) is used as the standard. Bilirubin standard solutions may be prepared either in human serum or in 40 g/L albumin solution (human or bovine), because we found the molar absorptivity of the azopigment at 598 nm to be identical in these media. The absorptivities of the unconjugated and conjugated azopigments appear to be identical, but the conjugated azopigment is completely hydrolyzed in the final reaction mixture. Bilirubin added to serum from adults or neonates was quantitatively accounted for. Interference by hemoglobin (up to 2 g/L), ascorbic acid (up to 20 mg/L), or zinc (at physiological concentrations) is negligible. Of the therapeutic drugs we tested, only L-dopa and alpha-methyldopa interfere. We established normal adult reference values for total bilirubin and examined the intraindividual variation in 19 subjects.
这种用于测量血清总胆红素的候选参考方法基于Jendrassik-Gróf原理（《临床化学》29: 297 - 301, 1983）。使用916号胆红素标准参考物质（国家标准局）作为标准。胆红素标准溶液可以用人血清或40 g/L白蛋白溶液（人或牛）配制，因为我们发现偶氮色素在598 nm处的摩尔吸光系数在这些介质中是相同的。未结合和结合的偶氮色素的吸光系数似乎相同，但结合的偶氮色素在最终反应混合物中会完全水解。添加到成人或新生儿血清中的胆红素得到了定量测定。血红蛋白（高达2 g/L）、抗坏血酸（高达20 mg/L）或锌（生理浓度）的干扰可忽略不计。在我们测试的治疗药物中，只有左旋多巴和α-甲基多巴有干扰。我们确定了成人总胆红素的正常参考值，并检查了19名受试者的个体内变异。
