Cherenack Emily M, Larson Michaela E, Murray Kevin, Mayer Zachary J, Guerrero Candace, Broedlow Courtney A, Klatt Nichole R, Carrico Adam W
Department of Psychiatry and Behavioral Sciences, University of Miami Miller School of Medicine, Miami, FL, USA.
Robert Stempel College of Public Health and Social Work, Florida International University, Miami, FL, USA.
J Neuroimmune Pharmacol. 2025 Jun 19;20(1):68. doi: 10.1007/s11481-025-10223-4.
Metabolomics can be used to identify biological targets to mitigate the negative impacts of HIV and stimulant use on neuroimmune and cardiometabolic functioning. However, studies are needed to characterize the plasma metabolome among sexual minority men (SMM) in the context of independent and co-occurring HIV and stimulant use. From 2020 to 2022, we collected plasma samples and assessed biologically confirmed HIV status and stimulant use among 61 community-recruited SMM in Miami, Florida. Cross-sectional bivariate analyses and multivariable regressions correcting for false discovery rate compared 390 mass spectrometry-based plasma metabolites across four HIV/stimulant use groups: (1) living without HIV and no stimulant use (HIV-STIM-), (2) living with HIV and no stimulant use (HIV + STIM-), (3) living without HIV with stimulant use (HIV-STIM +), and (4) living with HIV with stimulant use (HIV + STIM +). Six metabolites showed differences between HIV/stimulant use groups at p < 0.05 in both Kruskal-Wallis tests and linear regressions: choline, tryptophan, two lysophosphatidylcholines, one triacylglyceride, and one dihexosylceramide. After correcting for false discovery rate, in linear regressions controlling for BMI and age, the HIV + STIM + group had lower aspartic acid than the HIV-STIM- group, higher lysophosphatidylcholine a C18:1 than the HIV-STIM + group, and higher triacylglyceride(20:3_34:0) than the HIV-STIM- and HIV + STIM- groups. In SMM, co-occurring stimulant use and HIV were associated with perturbations in certain metabolites. Metabolites such as aspartic acid and lysophosphatidylcholines are potentially involved in immune dysregulation, addiction, energy use, and cardiovascular disease. Trials of interventions to reduce stimulant use could elucidate its causal relationship to metabolites.
代谢组学可用于识别生物学靶点，以减轻艾滋病毒和使用兴奋剂对神经免疫和心脏代谢功能的负面影响。然而，需要开展研究来描述男同性恋者（SMM）在独立使用和同时使用艾滋病毒与兴奋剂情况下的血浆代谢组特征。2020年至2022年期间，我们收集了血浆样本，并评估了佛罗里达州迈阿密61名通过社区招募的男同性恋者经生物学确认的艾滋病毒感染状况和兴奋剂使用情况。横断面双变量分析和校正错误发现率的多变量回归比较了四个艾滋病毒/兴奋剂使用组中基于质谱的390种血浆代谢物：（1）未感染艾滋病毒且未使用兴奋剂（HIV-STIM-）；（2）感染艾滋病毒且未使用兴奋剂（HIV+STIM-）；（3）未感染艾滋病毒但使用兴奋剂（HIV-STIM+）；（4）感染艾滋病毒且使用兴奋剂（HIV+STIM+）。在Kruskal-Wallis检验和线性回归中，六种代谢物在艾滋病毒/兴奋剂使用组之间的差异均为p<0.05：胆碱、色氨酸、两种溶血磷脂酰胆碱、一种甘油三酯和一种二己糖神经酰胺。校正错误发现率后，在控制体重指数和年龄的线性回归中，HIV+STIM+组的天冬氨酸低于HIV-STIM-组，溶血磷脂酰胆碱a C18:1高于HIV-STIM+组，甘油三酯（20:3_34:0）高于HIV-STIM-组和HIV+STIM-组。在男同性恋者中，同时使用兴奋剂和感染艾滋病毒与某些代谢物的紊乱有关。天冬氨酸和溶血磷脂酰胆碱等代谢物可能参与免疫失调、成瘾、能量利用和心血管疾病。减少兴奋剂使用的干预试验可能会阐明其与代谢物之间的因果关系。
