Liska S K, Kerkay J, Pearson K H
Clin Chim Acta. 1985 Oct 15;151(3):231-6. doi: 10.1016/0009-8981(85)90085-3.
Zinc and copper were determined in urine using polarized Zeeman effect flame atomic absorption spectrophotometry. For the zinc assay, urine was diluted 1/10 with deionized water. Concentrations could be determined by comparison to standards in a salt matrix or in a commercial urine control. The linearity of the assay was 350 micrograms/l, the detection limit was 1.2 micrograms/l and the within-run relative standard deviation (RSD) was 2.08%, 3.06%, 0.71% and 1.29% for specimens with zinc concentrations of 202 micrograms/l, 206 micrograms/l, 1 003 micrograms/l and 1 032 micrograms/l, respectively. The between-run RSD was 2.34% for a mean zinc concentration of 461 micrograms/l. For the copper assay, urine was aspirated directly and concentrations were determined by standard additions. The linearity of the assay was 5 000 micrograms/l, the detection limit was 4.6 micrograms/l and the within-run RSD was 24.49%, 16.10%, 4.00% and 3.19% for specimens with copper concentrations of 9.8 micrograms/l, 11.8 micrograms/l, 50.0 micrograms/l and 50.2 micrograms/l, respectively. The between-run RSD was 8.78% and 4.72% for specimens with copper concentrations of 21.1 micrograms/l and 40.3 micrograms/l, respectively.
采用偏振塞曼效应火焰原子吸收分光光度法测定尿液中的锌和铜。对于锌的测定，尿液用去离子水稀释1/10。可通过与盐基质中的标准品或商业尿液对照品比较来确定浓度。该测定方法的线性范围为350微克/升，检测限为1.2微克/升，对于锌浓度分别为202微克/升、206微克/升、1003微克/升和1032微克/升的标本，批内相对标准偏差（RSD）分别为2.08%、3.06%、0.71%和1.29%。对于平均锌浓度为461微克/升的标本，批间RSD为2.34%。对于铜的测定，直接吸取尿液并通过标准加入法确定浓度。该测定方法的线性范围为5000微克/升，检测限为4.6微克/升，对于铜浓度分别为9.8微克/升、11.8微克/升、50.0微克/升和50.2微克/升的标本，批内RSD分别为24.49%、16.10%、4.00%和3.19%。对于铜浓度分别为21.1微克/升和40.3微克/升的标本，批间RSD分别为8.78%和4.72%。
