Choudhury Sriya, Saha Ishita, Bandyopadhyay Bidyut, Banerjee Priyajit
Laboratory of Drug Discovery and Protein Engineering, School of Life Sciences, Swami Vivekananda University, Barrackpore, West Bengal, India.
Department of Physiology, Medical College Kolkata, Kolkata, West Bengal, India.
Biotechnol Appl Biochem. 2025 Jun 18. doi: 10.1002/bab.70013.
Probiotics serve as essential wound healers, particularly in diabetic patients, where they eliminate the interfering hindrance caused due to enhanced oxidative stress, inflammation, and microbial imbalances. Probiotic strains are also reported to enhance the level of tissue regeneration-specific cytokines and growth factors. Similarly, probiotics also stop infections by preventing the growth of harmful bacteria at the lesion site, which may be helpful for susceptible diabetic ulcers. Thus, probiotics may be a viable adjuvant treatment to hasten the healing of diabetic wounds. The present study aims to evaluate the efficacy of probiotic and synbiotic supplementation on wound healing in diabetic mice. Accordingly, probiotic strains Bacillus cereus strain BWN SC and Bacillus aerophilus strain BWN SC were isolated from whey water and fermented rice water on MRS agar media. Their wound-healing potential was investigated in male Swiss albino mice. Initially, streptozotocin was used to induce diabetes in the animals, after which 3 mm subcutaneous puncture wounds were induced on the dorsal region. Next, they were treated with different probiotics or synbiotics applied topically on the wounded area. Simultaneously, oral supplementation of the respective strains was also done using gavage. Wound contraction rate was studied at intervals of 1, 5, 10, and 14 days, followed by subsequent histological assay and hematological analysis. Animals treated with probiotics and synbiotics supplementation showed a faster wound contraction rate as compared to the control. Hematological analysis also showed improved wound-healing parameters in the treated mice. Thus, isolated probiotic strains can be effectively used for wound healing in diabetic mice. They can be combined with prebiotics to get even more potent therapeutic tool in the form of synbiotics.
益生菌是重要的伤口愈合促进剂，尤其对于糖尿病患者，它们能消除因氧化应激增强、炎症和微生物失衡所造成的干扰阻碍。据报道，益生菌菌株还能提高组织再生特异性细胞因子和生长因子的水平。同样，益生菌通过阻止有害细菌在损伤部位生长来预防感染，这对易感染的糖尿病溃疡可能有帮助。因此，益生菌可能是加速糖尿病伤口愈合的一种可行辅助治疗方法。本研究旨在评估补充益生菌和合生元对糖尿病小鼠伤口愈合的疗效。相应地，从乳清和发酵米水中分离出蜡样芽孢杆菌菌株BWN SC和气生芽孢杆菌菌株BWN SC，并接种于MRS琼脂培养基上。在雄性瑞士白化小鼠中研究了它们的伤口愈合潜力。首先，用链脲佐菌素诱导动物患糖尿病，之后在背部区域造成3毫米的皮下穿刺伤口。接着，在伤口部位局部应用不同的益生菌或合生元进行治疗。同时，也通过灌胃对相应菌株进行口服补充。在第1、5、10和14天间隔研究伤口收缩率，随后进行组织学分析和血液学分析。与对照组相比，补充益生菌和合生元的动物伤口收缩速度更快。血液学分析还显示治疗小鼠的伤口愈合参数有所改善。因此，分离出的益生菌菌株可有效用于糖尿病小鼠的伤口愈合。它们可以与益生元结合，以合生元的形式获得更有效的治疗工具。
