Previtali Giulia, Seghezzi Michela, Marozzi Roberto, Fortino Monica, Agnolet Gianluca, Barretta Mauro, Bizzoni Claudia, Bolla Valeria, Bolzoni Greta, Cesani Alessia, Diambrini Matteo, Esposito Sara Apassiti, Giuliani Giorgia, Picciau Alina, Alessio Maria Grazia
Clinical Chemistry Laboratory, Department of Laboratory Medicine, Papa Giovanni XXIII Hospital, Bergamo, Italy.
Pract Lab Med. 2025 May 31;46:e00481. doi: 10.1016/j.plabm.2025.e00481. eCollection 2025 Sep.
The UF-1500 is the new fully automated urine particle analyser by Sysmex specifically tailored for small and medium laboratories. This study aim to validate its analytical and diagnostic performance.
754 first morning mid-stream urines were analysed on UF-1500; 550 samples were used for the UF-5000 comparison and 204 were used for the correlation with manual count on Fuchs-Rosenthal chamber. Carry-over, linearity and imprecision of the UF-1500 were also assessed.
Correlation with the UF-5000 was excellent, with coefficient range between 0.88 and 1.00. Correlation with Fuchs-Rosenthal chamber was very good for all the parameters; ranged between 0.67 and 0.94. Linearity regression coefficient of determination (R) was excellent for almost all the parameters. No carry-over was observed. The within-run imprecision range between 2.93 % for RBC and 35.63 % for WBC. The between-run imprecision ranged between 2.1 % for RBC and 23.9 % for CAST, using low and high positive quality controls, respectively.
The analytical and diagnostic performance is satisfactory for almost all the parameters, when compared with the UF-5000; the correlation with the reference method is equally good.
UF - 1500是Sysmex公司新推出的全自动尿液颗粒分析仪，专为中小型实验室量身定制。本研究旨在验证其分析和诊断性能。
对754份首次晨尿中段尿进行UF - 1500分析；550份样本用于与UF - 5000比较，204份用于与Fuchs - Rosenthal计数板手工计数进行相关性分析。还评估了UF - 1500的携带污染、线性和不精密度。
与UF - 5000的相关性极佳，相关系数范围在0.88至1.00之间。与Fuchs - Rosenthal计数板对所有参数的相关性都非常好；范围在0.67至0.94之间。几乎所有参数的线性回归决定系数（R）都很出色。未观察到携带污染。批内不精密度范围为红细胞2.93%至白细胞35.63%。分别使用低和高阳性质量控制品时，批间不精密度范围为红细胞2.1%至管型23.9%。
与UF - 5000相比，几乎所有参数的分析和诊断性能都令人满意；与参考方法的相关性同样良好。
