Tantisaranon Piraya, Dumkengkhachornwong Kanyarat, Aiadsakun Peechana, Hnoonual Areerat
Emergency Laboratory, Department of Pathology, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand.
Clinical Microscopy Laboratory, Department of Pathology, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand.
Pract Lab Med. 2021 Jan 18;24:e00203. doi: 10.1016/j.plabm.2021.e00203. eCollection 2021 Mar.
Microscopic examination is essential in urine analysis. This is a simple way to collect informative data but it is also labor-intensive, time-consuming, and requires experienced staff for accurate results and interpretation. Several automated urine analyzers have been introduced for urine analysis in medical laboratories. The aim of this study was to assess and compare the performance of the most common three automated urine analyzers, Cobas 6500, UN3000-111b and iRICELL 3000.
and Methods: A total of 100 routine urine samples were used in the study. Results from the three machines were compared with the routine procedure results including physical, chemical and sediment analysis.
There was good correlation of urine physical and chemical analyses between the three analyzers with an overall concordance level of more than 80%. For sediment analysis, the degree of concordance between manual analysis and the three instruments was very good to good for white blood cells, red blood cells and epithelial cells, and moderate for bacteria. There were fair to good agreements between manual microscopy and the three instruments, Cobas 6500, UN3000-111b and iRICELL 3000, for casts (Cohen's kappa 0.42, 0.38 and 0.62, respectively).
The three automated urine analyzers showed similar performances and good correlation with manual microscopy. The results of this study indicate that automated urine analyzers could be used for initial urine testing to reduce high workloads and to save time, but manual microscopic analysis by experienced staff is still necessary to classify urine sediments for confirmation, especially in pathologic specimens.
显微镜检查在尿液分析中至关重要。这是一种收集信息数据的简单方法，但它也是劳动密集型、耗时的，并且需要经验丰富的工作人员才能获得准确的结果和解释。医学实验室已经引入了几种自动化尿液分析仪用于尿液分析。本研究的目的是评估和比较三种最常用的自动化尿液分析仪Cobas 6500、UN3000 - 111b和iRICELL 3000的性能。
本研究共使用了100份常规尿液样本。将这三台机器的结果与包括物理、化学和沉渣分析在内的常规程序结果进行比较。
三台分析仪之间尿液物理和化学分析的相关性良好，总体一致性水平超过80%。对于沉渣分析，手工分析与这三台仪器在白细胞、红细胞和上皮细胞方面的一致性程度非常好至良好，在细菌方面为中等。手工显微镜检查与Cobas 6500、UN3000 - 111b和iRICELL 3000这三台仪器在管型方面的一致性为一般至良好（Cohen's kappa分别为0.42、0.38和0.62）。
这三台自动化尿液分析仪表现出相似的性能，与手工显微镜检查具有良好的相关性。本研究结果表明，自动化尿液分析仪可用于初步尿液检测，以减少高工作量并节省时间，但经验丰富的工作人员进行手工显微镜分析对于尿液沉渣分类确认仍然是必要的，尤其是在病理标本中。
