Chen Yiqiang, Jiang Xu, Zhao Juanjuan, Yang Maosheng, Chen Yong, Ling Hong, Liu Yang, Deng Feng, Wang Zhu
Key Laboratory of Environmental Remediation and Ecological Health, Ministry of Industry and Information Technology, Jiangsu Environmental Engineering Technology Co., Ltd, Jiangsu Environmental Protection Group Co., Ltd., Nanjing, China.
Institute of Environmental Research at Greater Bay, Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, Guangzhou University, Guangzhou, China.
Front Microbiol. 2025 Jun 4;16:1615317. doi: 10.3389/fmicb.2025.1615317. eCollection 2025.
This study investigated the responses of the bacterial community structure and metabolic pathways in a sulfur-based autotrophic denitrification filter (SADF) system to fast elevated sulfate salinity, from 0.04 to 1.2% in 30 days. Results showed that the SADF system exhibited robust sulfate salinity stress tolerance at low nitrate concentrations. In the context of sulfate scenarios, the genus significantly proliferated and was identified as the dominant sulfur-oxidizing player in the SADF system, achieving a relative abundance of 63.79% under 1.2% sulfate salinity. Cooperative and competitive interactions were found in the SADF-related microorganisms, promoting stable denitrification performance under high salinity. Surprisingly, with a low hydraulic retention time (HRT) of 60 min, metagenomic sequencing revealed a upregulated abundance of functional genes encoding for enzymes associated with nitrogen and sulfur metabolism, while positive correlations were observed between these two pathways in response to sulfate salinity. Furthermore, global wastewater treatment plants were thoroughly explored for the distribution of the SADF-related microorganisms identified in this study. Interestingly, one-way ANOVA analysis showed that the SADF-related microorganisms were widely distributed globally, demonstrating their universality in potential engineering applications worldwide.
本研究调查了基于硫的自养反硝化滤池（SADF）系统中细菌群落结构和代谢途径对硫酸盐盐度在30天内从0.04%快速升至1.2%的响应。结果表明，SADF系统在低硝酸盐浓度下表现出强大的硫酸盐盐度胁迫耐受性。在硫酸盐情景下，某属显著增殖，并被确定为SADF系统中主要的硫氧化菌，在1.2%的硫酸盐盐度下相对丰度达到63.79%。在与SADF相关的微生物中发现了协同和竞争相互作用，促进了高盐度下稳定的反硝化性能。令人惊讶的是，在60分钟的低水力停留时间（HRT）下，宏基因组测序显示编码与氮和硫代谢相关酶的功能基因丰度上调，同时在这两条途径之间观察到对硫酸盐盐度的正相关。此外，还对全球污水处理厂中本研究鉴定的与SADF相关微生物的分布进行了全面探索。有趣的是，单因素方差分析表明，与SADF相关的微生物在全球广泛分布，证明了它们在全球潜在工程应用中的普遍性。
