Bin Najeeb Mohd Aadam, Kamath Narayana, Kamath Shamika, Mistry Laresh N, Jaiswal Himmat, More Saudamini
Department of Microbiology, NAMO Medical Education and Research Institute, Silvassa, IND.
Department of Dentistry, NAMO Medical Education and Research Institute, Silvassa, IND.
Cureus. 2025 May 18;17(5):e84356. doi: 10.7759/cureus.84356. eCollection 2025 May.
Conjunctivitis, a common ocular condition, can be caused by various viral pathogens, leading to significant morbidity and occasional outbreaks. This study aimed to investigate the epidemiology, prevalence, molecular detection, and typing of viral pathogens in conjunctival specimens. A total of 450 conjunctival swabs were collected from patients presenting with conjunctivitis symptoms at Shri Vinoba Bhave Civil Hospital (SVBCH) between January 2024 and December 2024. Molecular techniques such as reverse transcription polymerase chain reaction (RT-PCR) and sequencing methods were employed to detect and characterize viral agents, including adenovirus, herpes simplex virus (HSV), enterovirus, and others. The overall prevalence of viral pathogens was found to be 69.3%, with adenovirus being the most prevalent, detected in 238 samples (52.9%), and identified as the dominant etiological agent. Molecular typing revealed the circulation of common serotypes, including Ad8 (n = 82, 39.0%), Ad19 (n = 54, 25.7%), Ad37 (n = 36, 17.1%), Ad5 (n = 22, 10.5%), and Ad11 (n = 16, 7.6%), providing insights into strain diversity and potential transmission patterns. Epidemiological analysis identified key risk factors, seasonal trends, and demographic associations. These findings enhance our understanding of the etiology of viral conjunctivitis, supporting improved diagnostic strategies, outbreak control, and public health interventions.
结膜炎是一种常见的眼部疾病，可由多种病毒病原体引起，导致较高的发病率并偶尔引发疫情。本研究旨在调查结膜标本中病毒病原体的流行病学、患病率、分子检测及分型。2024年1月至2024年12月期间，从施里·维诺巴·巴韦市民医院（SVBCH）出现结膜炎症状的患者中收集了共450份结膜拭子。采用逆转录聚合酶链反应（RT-PCR）等分子技术和测序方法来检测和鉴定病毒病原体，包括腺病毒、单纯疱疹病毒（HSV）、肠道病毒等。发现病毒病原体的总体患病率为69.3%，其中腺病毒最为常见，在238份样本（52.9%）中检测到，被确定为主要病原体。分子分型显示常见血清型的传播情况，包括Ad8（n = 82，39.0%）、Ad19（n = 54，25.7%）、Ad37（n = 36，17.1%）、Ad5（n = 22，10.5%）和Ad11（n = 16，7.6%），为毒株多样性和潜在传播模式提供了见解。流行病学分析确定了关键风险因素、季节性趋势和人口统计学关联。这些发现增进了我们对病毒性结膜炎病因的理解，有助于改进诊断策略、疫情控制和公共卫生干预措施。
