Kikuchi M, Nakao Y
J Antibiot (Tokyo). 1977 Mar;30(3):209-14. doi: 10.7164/antibiotics.30.209.
Some strains belonging to the genera Bacillus and Pseudomonas were found to produce in the culture broth, a protein which synergistically enhanced the activity of macrolide antibiotics against Pseudomonas aeroginosa. The protein produced by Bacillus brevis ATCC 8185, designated SP127, was isolated in a pure state by means of ammonium sulfate fractionation, CM-cellulose column chromatography and gel filtration on Sephadex G-100. The sedimentation coefficient and the molecular weight of SP127 were 1.8 s and 15,000, respectively according to the analytical ultracentrifuge. The isoelectric point of SP127, obtained by electrofocusing experiments, was over 10.0. Half cystine and histidine were absent in the molecule.
已发现芽孢杆菌属和假单胞菌属的一些菌株能在培养液中产生一种蛋白质，该蛋白质可协同增强大环内酯类抗生素对铜绿假单胞菌的活性。短短芽孢杆菌ATCC 8185产生的蛋白质命名为SP127，通过硫酸铵分级分离、CM - 纤维素柱色谱法以及Sephadex G - 100凝胶过滤法将其分离为纯品。根据分析超速离心结果，SP127的沉降系数和分子量分别为1.8 s和15,000。通过等电聚焦实验得到的SP127等电点超过10.0。该分子中不存在半胱氨酸和组氨酸。
