软骨发育不良中的软骨胶原蛋白分析。
Cartilage collagen analysis in the chondrodystrophies.
作者信息
Horton W A, Chou J W, Machado M A
出版信息
Coll Relat Res. 1985 Sep;5(4):349-54. doi: 10.1016/s0174-173x(85)80023-6.
PMID:4053564
Abstract
A simple and reproducible method for analyzing small samples of cartilage collagens was developed. Following extraction with guanidine HCl, the cartilage specimens were digested directly with CNBr and the resultant peptides separated by gel-permeation high-performance liquid chromatography. Resting cartilage collagen CNBr peptide maps differed from normal in two inherited chondrodystrophies, achondrogenesis II and spondyloepiphyseal dysplasia congenita.
摘要
开发了一种用于分析软骨胶原蛋白小样本的简单且可重复的方法。用盐酸胍提取后，软骨标本直接用溴化氰消化，所得肽通过凝胶渗透高效液相色谱分离。静止软骨胶原蛋白溴化氰肽图谱在两种遗传性软骨发育不良（II型软骨生成不全和先天性脊柱骨骺发育不良）中与正常情况不同。
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