通过高效液相色谱法分离胶原蛋白的溴化氰肽段。

Separation of cyanogen bromide peptides of collagen by means of high-performance liquid chromatography.

作者信息

Black C, Douglas D M, Tanzer M L

出版信息

J Chromatogr. 1980 Apr 4;190(2):393-400. doi: 10.1016/s0021-9673(00)88244-8.

Abstract

Cyanogen bromide peptides of bovine collagen Types I, II nad III were analyzed using high-performance liquid chromatography (HPLC). Elution patterns of each collagen type were unique and reproducible. Elution patterns of the CNBr peptides of the alpha 1 and alpha 2 chains of Type I collagen were also unique and together accounted for the major components of Type I collagen. Analysis of the eluted peptides from HPLC of each collagen type by sodium dodecyl sulphate-polyacrylamide gel electrophoresis showed specific patterns for each collagen. Thus, unique and reproducible HPLC chromatograms were obtained, providing a new analytical method that is simple, sensitive and rapid.

摘要

使用高效液相色谱法（HPLC）分析了牛I型、II型和III型胶原蛋白的溴化氰肽。每种胶原蛋白类型的洗脱模式都是独特且可重复的。I型胶原蛋白α1和α2链的CNBr肽的洗脱模式也很独特，共同构成了I型胶原蛋白的主要成分。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳对每种胶原蛋白类型的HPLC洗脱肽进行分析，显示出每种胶原蛋白的特定模式。因此，获得了独特且可重复的HPLC色谱图，提供了一种简单、灵敏且快速的新分析方法。