Vernon R G, Finley E, Taylor E
Comp Biochem Physiol B. 1985;82(1):133-6. doi: 10.1016/0305-0491(85)90141-5.
The rates of incorporation of 14C from 14C labelled acetate, glucose, alanine, leucine, isoleucine and valine into fatty acids has been measured in perirenal adipose tissue from foetal lambs and 8-month-old sheep, and into both fatty acids and acylglycerol glycerol in adipose tissue from 3-year-old sheep and 220-240 g female rats. Rates of incorporation of 14C from amino acids into fatty acids were much lower in adipose tissue from sheep (at all three ages) than from rats, whereas rates of incorporation of 14C into acylglycerol glycerol were either greater in sheep adipose tissue or the same as in rat adipose tissue. The rate of incorporation of 14C from amino acids into fatty acids decreased in the order leucine greater than alanine greater than isoleucine greater than valine in adipose tissue from rats and foetal lambs, and in the order leucine greater than alanine = isoleucine greater than valine in adipose tissue from 8-month- and 3-year-old sheep. Amino acids make a very small contribution to fatty acid synthesis in adipose tissue from sheep at all stages of development examined while fatty acids are a minor product of amino acid metabolism in sheep adipose tissue. The study provides further evidence for an important role for ATP-citrate lyase in restricting the utilization of acetyl-CoA generated in the mitochondria for fatty acid synthesis.
已测定了来自胎羊和8月龄绵羊肾周脂肪组织中，14C从14C标记的乙酸盐、葡萄糖、丙氨酸、亮氨酸、异亮氨酸和缬氨酸掺入脂肪酸的速率，以及来自3岁绵羊和220 - 240克雌性大鼠脂肪组织中，14C掺入脂肪酸和酰基甘油甘油的速率。在绵羊（所有三个年龄段）的脂肪组织中，氨基酸的14C掺入脂肪酸的速率远低于大鼠，而14C掺入酰基甘油甘油的速率在绵羊脂肪组织中要么更高，要么与大鼠脂肪组织相同。在大鼠和胎羊的脂肪组织中，氨基酸的14C掺入脂肪酸的速率按亮氨酸＞丙氨酸＞异亮氨酸＞缬氨酸的顺序降低，在8月龄和3岁绵羊的脂肪组织中按亮氨酸＞丙氨酸 = 异亮氨酸＞缬氨酸的顺序降低。在所研究的绵羊发育的所有阶段，氨基酸对脂肪组织中脂肪酸合成的贡献非常小，而脂肪酸是绵羊脂肪组织中氨基酸代谢的次要产物。该研究进一步证明了ATP - 柠檬酸裂解酶在限制线粒体中产生的乙酰辅酶A用于脂肪酸合成的利用方面的重要作用。
