A miR-JL-5 inhibitor modulates the proliferation of e in .

BACKGROUND AND AIMS

is the native host of (). Previous studies reported that was more harmful to the new host () than the original host that had spread globally and become one of the factors implicated in honeybee colony collapses. Therefore, it was essential to study the relationship between and for the prevention and control of the disease.

METHODS

In order to effectively block the regulation of spore proliferation in honeybees, we designed a miR-JL-5 inhibitor. In this study, infected bees () were fed sugar water with miR-JL-5 inhibitor in incubators using RNA interference technology. The spores load and the expression of microRNA, DEmRNA, and target gene among miRNA inhibitor group, infection group, and miRNA scramble group were compared. Both the biological functions of miR-JL-5 and the target gene of miRNAJL-5, were verified using RNA interference.

RESULTS

Our results showed that expression levels of JL-5 and the spore load in the miRNA inhibitor group were significantly lower than those in the infection and miRNA-scramble groups. We predicted that 847 honeybee genes and 133 N genes can be targeted by the miR-JL-5. Whole transcriptome sequencing results showed that a total of 5 honeybee DEmRNAs, and 0 N DEmRNA were identified in miRNA inhibitor group, infection group, and miRNA scramble group, in which only the honeybee mucin-19-like gene was the target gene by miR-JL-5.

CONCLUSION

These findings reveal that feeding miR-JL-5 inhibitor could reduce levels by altering the expression of miR-JL-5 and related target gene (honeybee mucin-19-like gene). Our results provide insights into role that microRNA regulates the proliferation of in .