Hu Congwu, Dong Zhanqi, Deng Boyuan, Wu Qin, Chen Peng, Lu Cheng, Pan Minhui
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China.
Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, Southwest University, Chongqing 400716, China.
J Fungi (Basel). 2021 Dec 8;7(12):1051. doi: 10.3390/jof7121051.
As microRNAs (miRNAs) are important expression regulators of coding RNA, it is important to characterize their role in the interaction between hosts and pathogens. To obtain a comprehensive understanding of the miRNA alternation in () infected with (), RNA sequencing and stem-loop qPCR were conducted to screen and identify the significantly differentially expressed miRNAs (DEmiRNAs). A total of 17 such miRNAs were identified in response to infection, among which miR6498-5p efficiently inhibited the proliferation of in BmE-SWU1 (BmE) cells by downregulating (). In addition, a fluorescence in situ hybridization (FISH) assay showed that miR6498-5p was located in the cytoplasm of BmE cells, while it was not found in the schizonts of . Further investigation of the effect of on the proliferation of schizonts found that the positive factor could facilitate completing its life cycle in cells by overexpression and RNAi of . Our findings offer multiple new insights into the role of miRNAs in the interaction between hosts and microsporidia.
由于微小RNA（miRNA）是编码RNA的重要表达调节因子，因此表征它们在宿主与病原体相互作用中的作用很重要。为了全面了解感染了（）的（）中miRNA的变化，进行了RNA测序和茎环定量PCR，以筛选和鉴定显著差异表达的miRNA（DEmiRNA）。总共鉴定出17种响应感染的此类miRNA，其中miR6498-5p通过下调（）有效抑制了BmE-SWU1（BmE）细胞中的（）增殖。此外，荧光原位杂交（FISH）分析表明，miR6498-5p位于BmE细胞的细胞质中，而在（）的裂殖体中未发现。对（）对裂殖体增殖影响的进一步研究发现，阳性因子（）可通过（）的过表达和RNA干扰促进（）在细胞中完成其生命周期。我们的研究结果为miRNA在宿主与微孢子虫相互作用中的作用提供了多个新见解。
