Robertson N P, Cain G D
Comp Biochem Physiol B. 1985;82(2):299-306. doi: 10.1016/0305-0491(85)90245-7.
Tegumental tissues of paired adult Schistosoma mansoni were removed by treatment with Triton X-100 and recovered by centrifugation. The chloroform-methanol insoluble residues of this isolated tegumental fraction and of the denuded carcasses were analysed for glycosaminoglycan (GAG) and sialic acid contents. Treatment with GAG-specific enzymes followed by electrophoretic analysis showed that both the carcass and tegument contained heparin and/or heparan sulfate, chondroitin sulfate and hyaluronic acid. All these except hyaluronic acid were present in the tegumental fraction. Based on uronic acid content, about 73% of the total GAG was in the tegumental membrane, 15% in the tegmental matrix and the remaining 12% was in the carcass. The presence of heparin-like polysaccharide may present entrapment of the schistosoma by the hosts' blood-clotting process.
成年曼氏血吸虫的成对体表组织经Triton X-100处理后去除，并通过离心回收。对该分离的体表部分和去壳虫体的氯仿-甲醇不溶性残渣进行糖胺聚糖（GAG）和唾液酸含量分析。用GAG特异性酶处理后进行电泳分析表明，虫体和体表均含有肝素和/或硫酸乙酰肝素、硫酸软骨素和透明质酸。除透明质酸外，所有这些都存在于体表部分。根据糖醛酸含量，约73%的总GAG存在于体表膜中，15%存在于体表基质中，其余12%存在于虫体中。肝素样多糖的存在可能导致宿主凝血过程对血吸虫的捕获。
