Robertson N P, Cain G D
Mol Biochem Parasitol. 1984 Jun;12(2):173-83. doi: 10.1016/0166-6851(84)90133-6.
The teguments of 6 and 10 day-old Hymenolepis diminuta were removed with Triton X-100 and separated into brush border and vesicular fractions by differential centrifugation. Glycosaminoglycans (GAG) isolated from these tissues and from the denuded carcass were treated with specific GAG-degrading enzymes and other chemical agents and analyzed by sodium dodecyl sulfate-polyacrylamide, agarose gel and cellulose acetate electrophoresis. Both 6 and 10 day-old worm carcasses contained chondroitin sulfate, heparin/heparan sulfate and hyaluronic acid. The 10 day-old worm brush border and vesicle fractions contained chondroitin sulfate but no heparin-like material. Colorimetric analysis showed that the carcasses of both 6 and 10 day-old worms contained uronic acid. About 98% of the detectable uronic acid of 10 day-old worms was found in the carcass, and only 2% in the brush border fraction. No uronic acid was detected in the other tegumental fractions.
用曲拉通X-100去除6日龄和10日龄微小膜壳绦虫的皮层，并通过差速离心将其分离为刷状缘和囊泡部分。从这些组织和去皮虫体中分离出的糖胺聚糖（GAG）用特定的GAG降解酶和其他化学试剂处理，并通过十二烷基硫酸钠-聚丙烯酰胺、琼脂糖凝胶和醋酸纤维素电泳进行分析。6日龄和10日龄的虫体均含有硫酸软骨素、肝素/硫酸乙酰肝素和透明质酸。10日龄虫体的刷状缘和囊泡部分含有硫酸软骨素，但不含类肝素物质。比色分析表明，6日龄和10日龄虫体的虫体均含有糖醛酸。在10日龄虫体中，约98%的可检测糖醛酸存在于虫体中，仅2%存在于刷状缘部分。在其他皮层部分未检测到糖醛酸。
