维生素B12抗代谢物的微生物生产。III. 来自蜡样芽孢杆菌的化合物102804

Microbial production of vitamin B12 antimetabolites. III Compound 102804 from Bacillus cereus.

作者信息

Kageyama M, Burg K A, Perlman D

出版信息

J Antibiot (Tokyo). 1977 Apr;30(4):283-8. doi: 10.7164/antibiotics.30.283.

DOI:10.7164/antibiotics.30.283

Abstract

A basic compound with empirical formula C12H16N2O5 was isolated from Bacillus cereus 102804 fermentations of a soybean meal-glucose medium. The inhibitory activity of compound 102804 on growth of Gram-positive and Gram-negative bacteria growing in a chemically defined medium was reversed by vitamin B12, by L-methionine, and by D-methionine. It has no inhibitory activity for Escherichia coli (Davis 113-3) when grown in media containing L-methionine. The biosynthesis of 102804 coincides with the sporulation of the B. cereus, and the compound is not produced in the absence of sporulation.

摘要

从蜡样芽孢杆菌102804在豆粕-葡萄糖培养基中的发酵产物中分离出一种经验式为C12H16N2O5的碱性化合物。化合物102804对在化学成分明确的培养基中生长的革兰氏阳性菌和革兰氏阴性菌生长的抑制活性可被维生素B12、L-甲硫氨酸和D-甲硫氨酸逆转。当在含有L-甲硫氨酸的培养基中生长时，它对大肠杆菌（Davis 113-3）没有抑制活性。102804的生物合成与蜡样芽孢杆菌的孢子形成同时发生，并且在没有孢子形成的情况下不产生该化合物。