Wu Jiali, Nan Xiongxiong, Qin Ken, Dai Guoli, Zhang Xin, Yang Zijun, Wang Zhonghua, Wang Cuiping
School of Biological Science and Engineering, North Minzu University, Yinchuan, 750021, Ningxia, China.
State Key Laboratory of Efficient Production of Forest Resources, Yinchuan, 750002, China.
Planta. 2025 Jun 19;262(2):33. doi: 10.1007/s00425-025-04753-7.
Goji displays characteristics of gametophytic self-incompatibility. The S-RNase gene, expressed in the style, serves as the S determinant in the pistil, playing a key role in regulating goji's self-incompatibility. Goji, a plant commonly found worldwide, has been traditionally used for medicinal and culinary purposes in Chinese culture. However, breeding this species is challenging due to its self-incompatibility (SI). This study utilized 'Ningqi 1', 'Ningqi 6', and 'Ningqi 8', which exhibit significant variations in SI, as the experimental subjects. Detailed observations of floral organs and artificial pollination trials were conducted to elucidate the SI traits among different goji cultivars. Furthermore, the crucial pistil S factor that mediates SI in goji has been successfully cloned and subjected to analysis. The study revealed that goji exhibits gametophytic self-incompatibility (GSI). 'Ningqi 1' has a facultative selfing breeding system, while 'Ningqi 6' and 'Ningqi 8' involve facultative cross-pollination. Four S-RNase genes were successfully cloned from goji plants. Differential expression analysis revealed exclusive expression of S-RNase genes in the style. 'Ningqi 8' had significantly higher expression of the S-RNase gene compared to the S-RNase gene, suggesting that the S-RNase gene may play a pivotal role in regulating the SI mechanism of 'Ningqi 8'. This research provides insights into the cytological mechanisms of SI in goji, informing the planning of pollination tree arrangements and the selection of parental stocks for self-compatible breeding programs. It also lays the groundwork for future molecular studies on SI in goji.
枸杞表现出配子体自交不亲和的特征。在花柱中表达的S-RNase基因是雌蕊中的S决定因子，在调控枸杞的自交不亲和中起关键作用。枸杞是一种在全球广泛分布的植物，在中国文化中传统上用于药用和烹饪。然而，由于其自交不亲和性，该物种的育种具有挑战性。本研究以在自交不亲和性上表现出显著差异的‘宁杞1号’、‘宁杞6号’和‘宁杞8号’为实验对象。对花器官进行了详细观察并开展人工授粉试验，以阐明不同枸杞品种间的自交不亲和特性。此外，介导枸杞自交不亲和的关键雌蕊S因子已成功克隆并进行了分析。研究表明枸杞表现出配子体自交不亲和（GSI）。‘宁杞1号’具有兼性自交育种系统，而‘宁杞6号’和‘宁杞8号’涉及兼性异花授粉。从枸杞植株中成功克隆出四个S-RNase基因。差异表达分析表明S-RNase基因在花柱中特异性表达。与S-RNase基因相比，‘宁杞8号’的S-RNase基因表达显著更高，表明S-RNase基因可能在调控‘宁杞8号’的自交不亲和机制中起关键作用。本研究为枸杞自交不亲和的细胞学机制提供了见解，为授粉树配置规划和自交亲和育种计划的亲本选择提供了参考。它也为未来枸杞自交不亲和的分子研究奠定了基础。
