Baptista Rodrigo de Paula, Xiao Rui, Li Yiran, Glenn Travis C, Kissinger Jessica C
Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA, 30602, USA.
Institute of Bioinformatics, University of Georgia, Athens, GA, 30602, USA.
Sci Data. 2025 Jun 19;12(1):1039. doi: 10.1038/s41597-025-05364-3.
Cryptosporidium parvum is a significant pathogen causing gastrointestinal infections in humans and animals. It is spread through ingesting contaminated food and water. Despite its global health significance, generating a C. parvum genome sequence has been challenging for many reasons including cloning and challenging subtelomeric regions. A new, gapless, hybrid, telomere-to-telomere genome assembly was created for C. parvum IOWA II, here termed CpBGF. It reveals 8 chromosomes, a genome size of 9,259,183 bp, and resolves complex subtelomeric regions. To facilitate ease of use and consistency with the literature, the chromosomes have been oriented, and genes in this annotation have been given similar gene IDs as those used in the 2004, C. parvum IOWA II reference genome sequence. The new annotation utilized considerable RNA expression evidence including single-molecule Iso-Seq data; thus, untranslated regions, long noncoding RNAs, and antisense RNAs are annotated. The CpBGF genome assembly serves as a valuable resource for understanding the biology, pathogenesis, and transmission of C. parvum, and it facilitates the development of diagnostics, drugs, and vaccines against cryptosporidiosis.
微小隐孢子虫是一种在人类和动物中引起胃肠道感染的重要病原体。它通过摄入受污染的食物和水传播。尽管其对全球健康具有重要意义，但由于多种原因，包括克隆和具有挑战性的亚端粒区域，生成微小隐孢子虫的基因组序列一直具有挑战性。本文为微小隐孢子虫IOWA II创建了一个新的、无间隙的、混合的、端粒到端粒的基因组组装体，这里称为CpBGF。它揭示了8条染色体，基因组大小为9,259,183 bp，并解析了复杂的亚端粒区域。为便于使用并与文献保持一致，已对染色体进行了定向，并且本注释中的基因已被赋予与2004年微小隐孢子虫IOWA II参考基因组序列中使用的基因ID相似的基因ID。新的注释利用了大量的RNA表达证据，包括单分子Iso-Seq数据；因此，对非翻译区、长链非编码RNA和反义RNA进行了注释。CpBGF基因组组装体是了解微小隐孢子虫的生物学、发病机制和传播的宝贵资源，并且它有助于开发针对隐孢子虫病的诊断方法、药物和疫苗。
