Ren Yunxin, Chen Xi, Tang Cheng, Yue Hua
College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, China.
Front Vet Sci. 2025 Jun 5;12:1598013. doi: 10.3389/fvets.2025.1598013. eCollection 2025.
Bovine parainfluenza virus type 3 (BPIV3) is a significant pathogen responsible for bovine respiratory disease complex (BRDC) and reproductive disorders. Despite its importance, genomic studies of BPIV3 in abortion samples are currently lacking. We detected a BPIV3-positive sample via PCR in the lung sample from an aborted Holstein fetus; therefore, this study aimed to isolate and identify a BPIV3 strain from the aborted fetus sample of Holstein and analyze its genome. We successfully isolated and obtained a BPIV3 strain using MDBK cell lines, named LC1. Phylogenetic analysis based on complete genome sequences revealed that the LC1 strain belongs to genotype C and clusters into a branch with the Chinese strain SD2020. Notably, unique amino acid mutations were identified in the P (I297T) and M (P5N) proteins of the LC1 strain. To the best of our knowledge, this is the first BPIV3 genotype C strain to be isolated from a bovine abortion sample, as well as the first characterization of the abortion source BPIV3 genome reported. This finding lays the foundation for further studies on the genetic diversity of BPIV3.
牛副流感病毒3型（BPIV3）是引起牛呼吸道疾病综合征（BRDC）和生殖障碍的重要病原体。尽管其具有重要性，但目前缺乏对流产样本中BPIV3的基因组研究。我们通过PCR在一头流产的荷斯坦胎儿的肺样本中检测到一份BPIV3阳性样本；因此，本研究旨在从荷斯坦流产胎儿样本中分离并鉴定一株BPIV3毒株，并分析其基因组。我们使用MDBK细胞系成功分离并获得了一株BPIV3毒株，命名为LC1。基于全基因组序列的系统发育分析表明，LC1毒株属于C基因型，并与中国毒株SD2020聚类在一个分支中。值得注意的是，在LC1毒株的P（I297T）和M（P5N）蛋白中鉴定出了独特的氨基酸突变。据我们所知，这是首次从牛流产样本中分离出BPIV3 C基因型毒株，也是首次报道对流产源BPIV3基因组的特征分析。这一发现为进一步研究BPIV3的遗传多样性奠定了基础。
