Osborn D, Hamilton T C
J Cell Physiol. 1977 Jun;91(3):409-16. doi: 10.1002/jcp.1040910311.
Electron microprobe analyses of calcium distribution in the ciliated protozoan, Spirostomum ambiguum, indicated several calcium rich sites. One site was an endoplasmic distribution of calcium coincident with phosphorus which corroborates previous findings of hydroxyapatite deposits within Spirostomum. These apatite deposits were distributed throughout the endoplasm, but not within the nuclei or the contractile vacuole. Calcium was also detected within the cortical region. Cortical calcium was in greater concentration in the anterior portion of the organism and decreased towards the posterior end (region containing the contractile vacuole). Phosphorus and potassium were also detected as gradients from the anterior end, whereas magnesium was detected in the same density throughout the cortical region. Line scans of cortical regions suggested (1) distributions of calcium within mitochondria and/or vesicles, and (2) calcium associated with bundles of microfilaments.
对纤毛原生动物模糊旋口虫钙分布的电子微探针分析表明有几个富含钙的部位。一个部位是钙与磷一致的内质分布，这证实了先前在模糊旋口虫内发现的羟基磷灰石沉积物。这些磷灰石沉积物分布于整个内质，但不在细胞核或收缩泡内。在皮层区域也检测到了钙。皮层钙在生物体前部浓度较高，向后端（含收缩泡的区域）降低。还检测到磷和钾从前部呈梯度分布，而镁在整个皮层区域密度相同。皮层区域的线扫描表明：（1）钙在线粒体和/或小泡内的分布；（2）钙与微丝束相关。
