Tang Siqiong, Mu Kaiqin, Liang Xinqi, Su Jie, Zhang Jianping
College of Agriculture, Key Laboratory of Oasis Agricultural Pest Management and Plant Protection Resources Utilization, Xinjiang Uygur Autonomous Region, Shihezi University, Shihezi, Xinjiang 832003, China.
College of Agriculture, Key Laboratory of Oasis Agricultural Pest Management and Plant Protection Resources Utilization, Xinjiang Uygur Autonomous Region, Shihezi University, Shihezi, Xinjiang 832003, China.
Comp Biochem Physiol Part D Genomics Proteomics. 2025 Jun 19;56:101565. doi: 10.1016/j.cbd.2025.101565.
Neoseiulus bicaudus is a beneficial predatory mite used for the control of spider mites. Temperature is a crucial factor that influences the distribution, growth, and development of N. bicaudus. Cold acclimation is an important arthropod strategy used to improve cold tolerance. We investigated the impact of cold acclimation on the cold tolerance of N. bicaudus. To gain insights into the molecular mechanisms underlying cold acclimation of N. bicaudus, we conducted transcriptome and proteomic analyses on three cold-acclimated groups (6-h: 3 °C for 6 h; 24-h: 3 °C for 24 h; 7-day: 9 °C for 7 d). Cold acclimation, especially in the 7-day treatment, significantly improved the survival time of N. bicaudus at an acute low temperature (-6 °C). Multi-omics analysis revealed that cold acclimation in N. bicaudus involves coordinated regulation of genes and proteins related to energy metabolism and cellular protection. Cold acclimation suppressed energy-intensive pathways like fatty acid synthesis and glycolysis, reducing energy expenditure. However, it enhanced expression of proteins in fatty acid oxidation, tricarboxylic acid cycle, and oxidative phosphorylation pathways to maintain energy balance. Moreover, cold acclimation upregulated genes and proteins involved in mRNA processing, transport, translation regulation, protein folding, and degradation, ensuring rapid repair and synthesis of proteins for homeostasis. RNA interference of NbHSP70 and NbHSP90 showed that these genes play a vital role in regulating the cold tolerance of N. bicaudus. These findings provide valuable resources and opportunities to uncover molecular acclimation mechanisms that support cold tolerance in Phytoseiid mites.
双尾新小绥螨是一种用于控制叶螨的有益捕食螨。温度是影响双尾新小绥螨分布、生长和发育的关键因素。冷驯化是节肢动物用于提高耐寒性的重要策略。我们研究了冷驯化对双尾新小绥螨耐寒性的影响。为了深入了解双尾新小绥螨冷驯化的分子机制，我们对三个冷驯化组（6小时：3℃处理6小时；24小时：3℃处理24小时；7天：9℃处理7天）进行了转录组和蛋白质组分析。冷驯化，尤其是7天处理，显著提高了双尾新小绥螨在急性低温（-6℃）下的存活时间。多组学分析表明，双尾新小绥螨的冷驯化涉及与能量代谢和细胞保护相关的基因和蛋白质的协同调控。冷驯化抑制了脂肪酸合成和糖酵解等高能量消耗途径，减少了能量消耗。然而，它增强了脂肪酸氧化、三羧酸循环和氧化磷酸化途径中蛋白质的表达，以维持能量平衡。此外，冷驯化上调了参与mRNA加工、运输、翻译调控、蛋白质折叠和降解的基因和蛋白质，确保快速修复和合成蛋白质以维持体内平衡。对NbHSP70和NbHSP90进行RNA干扰表明，这些基因在调节双尾新小绥螨的耐寒性方面起着至关重要的作用。这些发现为揭示支持植绥螨耐寒性的分子驯化机制提供了宝贵的资源和机会。
