Hong Shuang, Liu Chengbin, Liu Xiaoyu, Rao Qinxiong, Wang Xianli, Zhang Qicai, Yao Chunxia, Song Weiguo
Institute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China; College of Fisheries and Life Science, Shanghai Ocean university, Shanghai 201306, China.
Institute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
Comp Biochem Physiol Part D Genomics Proteomics. 2025 Jun 19;56:101548. doi: 10.1016/j.cbd.2025.101548.
Perfluorooctane sulfonate (PFOS), a persistent organic pollutant, poses significant threats to aquatic ecosystems and organisms. This study investigated the biological effects of environmentally relevant PFOS concentrations (0 ng/L, 1 ng/L, 10 μg/L) on Cherax quadricarinatus, a freshwater crayfish of economic importance, focusing on histopathology, apoptosis (via TUNEL staining), and metabolomic alterations. The results showed that PFOS exposure caused tissue damage to the hepatopancreas of C. quadricarinatus, and PFOS exposure caused apoptosis of C. quadricarinatus hepatopancreas. In addition, the metabolomics results showed that there were several significant differential metabolites and metabolic pathways, which indicated that PFOS exposure can cause metabolic disorders in the hepatopancreas of C. quadricarinatus. The Starch and sucrose metabolism pathway was significantly enriched in both groups, suggesting the potential role of this pathway in the biological effects of PFOS on C. quadricarinatus. In addition, in 1 ng/L group, four significant differential metabolic pathways: Glucagon signaling pathway, Glycerolipid metabolism, Starch and sucrose metabolism, and Glycolysis/Gluconeogenesis were found, which demonstrated the adverse effects of PFOS even at environmental relevant concentration. Furthermore, in all the four metabolic pathways, glucose-1-phosphate is the only mutual significant differential metabolite, indicating that the carbohydrate metabolism might be the target affecting pathway and the glucose-1-phosphate assumed an important role in the metabolic effect of environmental relevant concentration of PFOS. This study revealed the effect of PFOS on C. quadricarinatus through tissue physiology and combined with metabolomics, which is of great significance for exploring the practical biological effects and health risk of PFOS in the actual aquatic environment.
全氟辛烷磺酸（PFOS）是一种持久性有机污染物，对水生生态系统和生物构成重大威胁。本研究调查了环境相关浓度的PFOS（0纳克/升、1纳克/升、10微克/升）对经济意义重大的淡水小龙虾四脊滑螯虾的生物学效应，重点关注组织病理学、凋亡（通过TUNEL染色）和代谢组学变化。结果表明，PFOS暴露导致四脊滑螯虾肝胰腺组织损伤，且PFOS暴露导致四脊滑螯虾肝胰腺细胞凋亡。此外，代谢组学结果显示存在几种显著差异代谢物和代谢途径，这表明PFOS暴露可导致四脊滑螯虾肝胰腺代谢紊乱。两组中淀粉和蔗糖代谢途径均显著富集，表明该途径在PFOS对四脊滑螯虾的生物学效应中可能发挥作用。此外，在1纳克/升组中，发现了四个显著差异代谢途径：胰高血糖素信号通路、甘油脂质代谢、淀粉和蔗糖代谢以及糖酵解/糖异生，这表明即使在环境相关浓度下PFOS也具有不利影响。此外，在所有这四个代谢途径中，葡萄糖-1-磷酸是唯一共同的显著差异代谢物，表明碳水化合物代谢可能是受影响的靶途径，且葡萄糖-1-磷酸在环境相关浓度PFOS的代谢效应中起重要作用。本研究通过组织生理学并结合代谢组学揭示了PFOS对四脊滑螯虾的影响，这对于探索PFOS在实际水生环境中的实际生物学效应和健康风险具有重要意义。
