Yawen Zong, Tiantian Shan, Yangyang Shi, Yiling Li, Siyue Lai, Yu Wei, Xiao Guo, Bolei Li, Biao Ren, Lei Cheng
State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China; Department of Operative Dentistry and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
Arch Oral Biol. 2025 Sep;177:106331. doi: 10.1016/j.archoralbio.2025.106331. Epub 2025 Jun 13.
This study aimed to evaluate the antimicrobial ability on the dual-species biofilm formed by C. albicans and E. faecalis and osteoclysis inhibition of DMAEM.
DMAEM monomer was used to evaluate the antmicrobial ability on the dual-species biofilm of C. albicans and E. faecalis by biomass quantification, Scanning electron microscope and confocal laser scanning microscopy scanning, and RT-qPCR. The biosafty and inhibition ability on the dual-species biofilm and osteoclysis of the experimental root canal sealers containing DMAEM was tested by biomass quantification, Cell Counting kit-8, tartrate-resistant acid phosphatase staining, flow cytometry analysis, and RT-qPCR.
The biomass of the dual-species biofilm formed by C. albicans and E. faecalis was significantly decreased by 26.3-51.3 % under 8-256 μg/mL DMAEM. 8 μg/mL DMAEM could reduce E. faecalis's survival rate to 77.4 % (p < 0.001) and down-regulated its virulence gene expression. Meanwhile, the mycelium count proportion of C. albicans in the dual-species biofilm was reduced from 50 % to 0 % (p < 0.0001). The experimental sealers with DMAEM content could also decrease the survival rate of C.albicans and E.faecalis in the dual-species biofilm. Moreover, the elution of experimental root canal sealers containing ≤ 2.5 % DMAEM was biosafe and improved 81.0 % (p < 0.05) osteoclastic inhibition compared with it of unmodified sealers at least.
The antimicrobial ability on the dual-species biofilm formed by C. albicans and E. faecalis and osteoclastic inhibition of DMAEM suggested its potential clinical application of DMAEM in the treatment of periapical inflammation.
本研究旨在评估二甲基丙烯酰乙胺（DMAEM）对白色念珠菌和粪肠球菌形成的双菌种生物膜的抗菌能力以及对骨吸收的抑制作用。
采用DMAEM单体，通过生物量定量、扫描电子显微镜和共聚焦激光扫描显微镜扫描以及逆转录-定量聚合酶链反应（RT-qPCR）来评估其对白色念珠菌和粪肠球菌双菌种生物膜的抗菌能力。通过生物量定量、细胞计数试剂盒-8、抗酒石酸酸性磷酸酶染色、流式细胞术分析和RT-qPCR，测试含DMAEM的实验性根管封闭剂对双菌种生物膜和骨吸收的生物安全性及抑制能力。
在8-256μg/mL的DMAEM作用下，白色念珠菌和粪肠球菌形成的双菌种生物膜的生物量显著降低了26.3%-51.3%。8μg/mL的DMAEM可将粪肠球菌的存活率降低至77.4%（p<0.001），并下调其毒力基因表达。同时，双菌种生物膜中白色念珠菌的菌丝计数比例从50%降至0%（p<0.0001）。含DMAEM的实验性封闭剂也可降低双菌种生物膜中白色念珠菌和粪肠球菌的存活率。此外，含≤2.5%DMAEM的实验性根管封闭剂的洗脱液具有生物安全性，与未改性的封闭剂相比，至少提高了81.0%（p<0.05）的破骨细胞抑制率。
DMAEM对白色念珠菌和粪肠球菌形成的双菌种生物膜的抗菌能力以及对骨吸收的抑制作用表明其在根尖周炎治疗中具有潜在的临床应用价值。
