In Vitro Antimicrobial Effects of Endoseal MTA and AH Plus Sealers on and Mature Biofilms.

This study compared the antimicrobial effects of Endoseal MTA and AH Plus sealers on () and () mature biofilms on dentin blocks. In this in vitro study, the root canals of 207 dentin blocks sectioned from the roots of extracted teeth were inoculated with and , and incubated for 3 weeks. The specimens were evaluated in 12 groups (n=17) for random root canal filling with AH Plus, Endoseal MTA, or no sealer, and assessment after 24 hours and 2 weeks. Dentin chips were cultured on blood agar, and the colonies were counted. One random specimen from each group was assessed under a scanning electron microscope (SEM). Data were analyzed using the Kruskal-Wallis and Mann-Whitney tests with Bonferroni adjustment at 0.05 level of significance. At 24 hours, Endoseal MTA significantly decreased the count compared with the positive control (P<0.008). At 2 weeks, the colony count of both microorganisms was significantly lower in the AH Plus group compared with the control group (P<0.008). The colony count in the Endoseal MTA group was significantly lower than that in the control group at 2 weeks (P<0.008). AH Plus caused significantly greater reduction in colony count at 2 weeks compared with Endoseal MTA (P<0.008). AH Plus showed a higher antimicrobial activity over time against both and . Endoseal MTA only showed short-term antibacterial effect.