Khoshbin Elham, Nikzad Forough, Arabestani Mohammad Reza, Farhdian Maryam, Karkehabadi Hamed
Endodontics Department, Dental Research Center, Avicenna Institute of Clinical Sciences, Avicenna Health Research Institute, Hamadan University of Medical Sciences, Hamadan, Iran.
Endodontics Department, School of Dentistry, Kashan University of Medical Sciences, Kashan, Iran.
Front Dent. 2025 May 19;22:19. doi: 10.18502/fid.v22i19.18760. eCollection 2025.
This study compared the antimicrobial effects of Endoseal MTA and AH Plus sealers on () and () mature biofilms on dentin blocks. In this in vitro study, the root canals of 207 dentin blocks sectioned from the roots of extracted teeth were inoculated with and , and incubated for 3 weeks. The specimens were evaluated in 12 groups (n=17) for random root canal filling with AH Plus, Endoseal MTA, or no sealer, and assessment after 24 hours and 2 weeks. Dentin chips were cultured on blood agar, and the colonies were counted. One random specimen from each group was assessed under a scanning electron microscope (SEM). Data were analyzed using the Kruskal-Wallis and Mann-Whitney tests with Bonferroni adjustment at 0.05 level of significance. At 24 hours, Endoseal MTA significantly decreased the count compared with the positive control (P<0.008). At 2 weeks, the colony count of both microorganisms was significantly lower in the AH Plus group compared with the control group (P<0.008). The colony count in the Endoseal MTA group was significantly lower than that in the control group at 2 weeks (P<0.008). AH Plus caused significantly greater reduction in colony count at 2 weeks compared with Endoseal MTA (P<0.008). AH Plus showed a higher antimicrobial activity over time against both and . Endoseal MTA only showed short-term antibacterial effect.
本研究比较了Endoseal MTA和AH Plus封闭剂对牙本质块上（）和（）成熟生物膜的抗菌效果。在这项体外研究中，将从拔除牙齿的牙根上切下的207个牙本质块的根管接种（）和（），并孵育3周。将标本分为12组（n = 17），随机用AH Plus、Endoseal MTA进行根管充填，或不使用封闭剂，在24小时和2周后进行评估。将牙本质碎屑接种于血琼脂培养基上培养，并计数菌落。每组随机选取一个标本在扫描电子显微镜（SEM）下进行评估。采用Kruskal-Wallis检验和Mann-Whitney检验对数据进行分析，并采用Bonferroni校正，显著性水平为0.05。在24小时时，与阳性对照组相比，Endoseal MTA显著降低了（）计数（P < 0.008）。在2周时，与对照组相比，AH Plus组两种微生物的菌落计数均显著降低（P < 0.008）。在2周时，Endoseal MTA组的（）菌落计数显著低于对照组（P < 0.008）。与Endoseal MTA相比，AH Plus在2周时导致（）菌落计数的降低更为显著（P < 0.008）。随着时间的推移，AH Plus对（）和（）均表现出更高的抗菌活性。Endoseal MTA仅表现出短期抗菌作用。
