Claycomb W C, Moses R L
Exp Cell Res. 1985 Nov;161(1):95-100. doi: 10.1016/0014-4827(85)90493-8.
Atrial and ventricular cardiac muscle cells isolated from the adult squirrel monkey Saimiri sciureus were cultured and characterized by light and transmission electron microscopy (TEM). Freshly isolated cells were striated and cylindrical in shape and, when placed in culture, rounded up and lost their highly organized morphology. With prolonged time in culture, they spread out on the surface of the culture flask and reacquired many of the internal ultrastructural characteristics of their in vivo atrial and ventricular cardiac muscle cell counterparts. Autoradiographic experiments indicated that both atrial and ventricular myocytes synthesized DNA when grown in culture. In some binucleated atrial cells only one nucleus became labelled. These studies show that it is now possible to culture cardiac muscle cells isolated from an adult primate.
从成年松鼠猴（Saimiri sciureus）分离出的心房和心室心肌细胞进行了培养，并通过光学显微镜和透射电子显微镜（TEM）进行了表征。刚分离出的细胞呈条纹状且呈圆柱形，当置于培养中时，会变圆并失去其高度有序的形态。随着培养时间的延长，它们在培养瓶表面铺展，并重新获得了其体内心房和心室心肌细胞对应物的许多内部超微结构特征。放射自显影实验表明，心房和心室肌细胞在培养中生长时都会合成DNA。在一些双核心房细胞中，只有一个细胞核被标记。这些研究表明，现在有可能培养从成年灵长类动物分离出的心肌细胞。
