Claycomb W C, Delcarpio J B, Guice S E, Moses R L
Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, New Orleans 70112.
In Vitro Cell Dev Biol. 1989 Dec;25(12):1114-20. doi: 10.1007/BF02621262.
Atrial and ventricular cardiac muscle cells isolated from 14- to 18-wk old fetal human hearts were grown in culture and characterized. Once established in culture the flattened cells contracted spontaneously and possessed differentiated ultrastructural characteristics including organized sarcomeres, intercalated discs, and transverse tubules with couplings. Atrial granules were present in the cultured atrial cells. Some cultured ventricular myocytes also contained electron-dense granules associated with Golgi cisternae, which were similar in size and appearance to atrial granules. The cultured ventricular myocytes divided and expressed the genes for thymidine kinase, histone H4, myosin heavy chain, muscle-specific creatine kinase, atrial natriuretic factor, and insulin-like growth factor II. These results establish that differentiated fetal human heart muscle cells can be cultured in sufficient quantities for biochemical, molecular, and morphological analyses.
从14至18周龄的人类胎儿心脏中分离出的心房和心室心肌细胞在培养中生长并进行了特性鉴定。一旦在培养中建立，扁平细胞会自发收缩，并具有分化的超微结构特征，包括有组织的肌节、闰盘和带有连接的横小管。培养的心房细胞中存在心房颗粒。一些培养的心室肌细胞也含有与高尔基池相关的电子致密颗粒，其大小和外观与心房颗粒相似。培养的心室肌细胞进行分裂，并表达胸苷激酶、组蛋白H4、肌球蛋白重链、肌肉特异性肌酸激酶、心房利钠因子和胰岛素样生长因子II的基因。这些结果表明，分化的人类胎儿心肌细胞可以大量培养，用于生化、分子和形态学分析。
