Schmidt W E, Mutt V, Carlquist M, Kratzin H, Conlon J M, Creutzfeldt W
FEBS Lett. 1985 Oct 28;191(2):264-8. doi: 10.1016/0014-5793(85)80021-1.
The isolation and primary structure of a novel gastrointestinal peptide, designated valosin, is described. The peptide was purified from porcine upper gut extracts using an HPLC and N-terminal sequence screening strategy which depends on chromatographic and structural characteristics as isolation criterion. The amino acid sequence of this peptide consists of 25 amino acid residues:
本文描述了一种名为缬酪肽的新型胃肠肽的分离及一级结构。该肽是从猪的上消化道提取物中利用高效液相色谱法(HPLC)和N端序列筛选策略纯化得到的,该策略依赖于色谱和结构特征作为分离标准。此肽的氨基酸序列由25个氨基酸残基组成: