犬胰分泌型胰蛋白酶抑制剂的纯化及完整氨基酸序列
Purification and complete amino acid sequence of canine pancreatic secretory trypsin inhibitor.
作者信息
Kikuchi N, Nagata K, Yoshida N, Tanaka T, Yamamoto M, Saitoh Y
出版信息
FEBS Lett. 1985 Oct 28;191(2):269-72. doi: 10.1016/0014-5793(85)80022-3.
PMID:4054311
Abstract
Pancreatic secretory trypsin inhibitor (PSTI) was purified from canine pancreatic juice by HPLC. Canine PSTI inhibited bovine trypsin activity stoichiometrically and strongly with a dissociation constant of below 10(-9) M. The amino acid sequence of canine PSTI was determined by conventional methods. It had one more amino acid residue at the amino-terminus than other mammalian PSTIs, i.e. human, porcine, bovine and ovine.
摘要
通过高效液相色谱法从犬胰液中纯化出胰腺分泌型胰蛋白酶抑制剂（PSTI）。犬PSTI以化学计量方式强烈抑制牛胰蛋白酶活性，解离常数低于10^(-9) M。采用常规方法测定了犬PSTI的氨基酸序列。与其他哺乳动物的PSTI（即人、猪、牛和羊的PSTI）相比，犬PSTI在氨基末端多一个氨基酸残基。
Zotero 插件