Chigure Gajanan M, Sharma Anil Kumar, Ajithkumar K G, Fular Ashutosh, Tayade Amol B, Kumar Rajesh, Jadhav Nitin D, Kumar Sachin, Gupta Suman, Nagar Gaurav, Ravindran Reghu, Kumar Satyanshu, Julliet Sanis, Sankar Muthu, Ghosh Srikanta
Entomology Laboratory, Division of Parasitology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, 243122, Uttar Pradesh, India; Department of Veterinary Parasitology, College of Veterinary and Animal Sciences (MAFSU, Nagpur), Parbhani-431402, Maharashtra, India.
Entomology Laboratory, Division of Parasitology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, 243122, Uttar Pradesh, India; School of Agriculture, Uttaranchal University, Dehradun, 248007, Uttarakhand, India.
Exp Parasitol. 2025 Jul;274:108969. doi: 10.1016/j.exppara.2025.108969. Epub 2025 Jun 19.
Ageratum conyzoides (Family: Asteraceae), a common herbaceous weed has a long history of use in traditional medicine. Rhipicephalus microplus, the cattle tick is distributed globally and causing significant economic losses to the animal husbandry sectors. Due to repeated applications of chemical acaricides to control infestation on animals, the tick species developed resistance to most of the acaricides. In the present study, to manage the problem of resistant ticks, the potentiality of β-caryophyllene (1-3) is explored for its anti-tick efficacy against three important tick species. The LC value of β-caryophyllene (1-3) against reference susceptible IVRI-I strain of R. microplus was 0.0284 % (95 % CI = 266.5-299.4) while against multi-acaricide resistant field isolates it was 0.0507 % (95 % CI = 491.4-523.0). The compound was 95 % efficacious against Hyalomma anatolicum and R. annulatus ticks. The anti-tick efficacy of the compound after oral administration at the dose rate of 7.5 mg per kg body weight was tested under challenged infestation conditions and more than 80 % efficacy was noted. In a separate experiment, a 73.3-97.7 % (mean efficacy = 77.6 %) efficacy of β-caryophyllene (1-3) was noted for three months which was comparable and not significantly lower than the efficacy (95.8 %) observed in the amitraz treatment group of animals but significantly higher as compared to the control group of animals. The present study was focused on the identification of new plant derived anti-tick molecule having the acaricidal capability for the management of resistant tick infestations on animals.
胜红蓟（菊科）是一种常见的草本杂草，在传统医学中有悠久的使用历史。微小牛蜱在全球分布，给畜牧业造成重大经济损失。由于反复使用化学杀螨剂来控制动物身上的蜱虫感染，蜱虫对大多数杀螨剂产生了抗性。在本研究中，为解决蜱虫抗性问题，探索了β-石竹烯（1-3）对三种重要蜱虫种类的抗蜱效果。β-石竹烯（1-3）对微小牛蜱参考敏感IVRI-I株的LC值为0.0284%（95%置信区间=266.5-299.4），而对多杀螨剂抗性的田间分离株为0.0507%（95%置信区间=491.4-523.0）。该化合物对小亚璃眼蜱和环形牛蜱的杀灭效果达95%。在受挑战感染条件下，测试了该化合物以每千克体重7.5毫克的剂量口服给药后的抗蜱效果，观察到超过80%的效果。在一项单独实验中，β-石竹烯（1-3）在三个月内的效果为73.3-97.7%（平均效果=77.6%），与动物双甲脒治疗组观察到的效果（95.8%）相当且无显著降低，但与动物对照组相比显著更高。本研究的重点是鉴定具有杀螨能力的新型植物源抗蜱分子，用于管理动物身上的抗性蜱虫感染。
