Xia Haoxue, Liu Tianbo, He Yaru, Guo Qitang, Wu Hanxiang, Tang Guangfei
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
China Tobacco Corporation Hunan Company, Changsha, 410000, China.
Sci Data. 2025 Jun 21;12(1):1053. doi: 10.1038/s41597-025-05351-8.
Rhizoctonia solani is a ubiquitously distributed soil-borne fungal pathogen that causes serious diseases in many plants worldwide. It attracts significant research attention due to its considerable economic importance in agricultural production. However, the limited availability of genome information has further impeded the development of new molecular-targeted control technologies. By utilizing Illumina short-read, PacBio HiFi long-read, and high-throughput chromosome conformation capture (Hi-C) sequencing technologies, we present a comprehensive and continuous chromosome-level assembly for R. solani. The final genome size is 40,801,261 bp, consisting of 23 contigs with a N50 of 2,529,230 bp. Hi-C data aids in anchoring the assembly onto 16 chromosomes. Additionally, the genome contains 16.17% (6,597,897 bp) repeat elements, including 10,698 protein-coding genes and 232 non-coding RNAs. The high-quality genome of R. solani not only provides valuable genomic information for further comprehending the fungal pathobiology and evolution, but also contributes to the development of scientific control strategies for disease prevention and control in agriculture.
立枯丝核菌是一种广泛分布的土传真菌病原体，在全球许多植物中引发严重病害。因其在农业生产中具有相当大的经济重要性，它吸引了大量的研究关注。然而，基因组信息的有限可用性进一步阻碍了新型分子靶向控制技术的发展。通过利用Illumina短读长测序、PacBio HiFi长读长测序和高通量染色体构象捕获（Hi-C）测序技术，我们对立枯丝核菌进行了全面且连续的染色体水平组装。最终基因组大小为40,801,261 bp，由23个重叠群组成，N50为2,529,230 bp。Hi-C数据有助于将组装结果锚定到16条染色体上。此外，该基因组包含16.17%（6,597,897 bp）的重复元件，包括10,698个蛋白质编码基因和232个非编码RNA。立枯丝核菌的高质量基因组不仅为进一步理解真菌病理生物学和进化提供了有价值的基因组信息，也有助于制定农业疾病预防和控制的科学策略。
