研究少量早产母乳中噬菌体组与脂质组之间相互关系的方案。
Protocol to study the inter-relationship between phageome and lipidome in low-volume preterm milk.
作者信息
Yew Wen C, Young Gregory R, Cheung William, Nelson Andrew, Berrington Janet E, Smith Darren L
机构信息
Faculty of Health and Life Sciences, Northumbria University, Newcastle upon Tyne NE1 8ST, UK.
Faculty of Health and Life Sciences, Northumbria University, Newcastle upon Tyne NE1 8ST, UK; Hub for Biotechnology in the Built Environment, Northumbria University, Newcastle upon Tyne NE1 8ST, UK; Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne NE1 7RU, UK.
出版信息
STAR Protoc. 2025 Jun 21;6(3):103917. doi: 10.1016/j.xpro.2025.103917.
Bacteriophages and lipids in human milk may benefit preterm infant health by modulating gut microbiomes. Here, we present a protocol for analyzing the phageome and lipidome in preterm milk using shotgun metagenomics and untargeted lipidomics approaches, respectively. We describe steps for extracting phages and lipids in low-volume milk, characterizing phageome using an in-house bioinformatic pipeline, and statistical analysis to correlate the phageome and lipidome. Finally, we detail an in vitro assay to examine the associations between fatty acid chain length and phage morphotype. For complete details on the use and execution of this protocol, please refer to Yew et al..
母乳中的噬菌体和脂质可能通过调节肠道微生物群而有益于早产儿健康。在此，我们分别介绍一种使用鸟枪法宏基因组学和非靶向脂质组学方法分析早产母乳中噬菌体组和脂质组的方案。我们描述了从少量母乳中提取噬菌体和脂质的步骤，使用内部生物信息学流程对噬菌体组进行表征，以及将噬菌体组和脂质组进行关联的统计分析。最后，我们详细介绍一种体外试验，以研究脂肪酸链长度与噬菌体形态型之间的关联。有关本方案使用和实施的完整详细信息，请参阅Yew等人的研究。
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