Li Teng, Liu Chang, Xi Zeyan, Li Na, Liao Liwen, Zhou Yulong, Li Yaobing, Pan Luqing
Fisheries College, Ocean University of China, Yushan Road 5, Qingdao, 266003, China.
Fisheries College, Ocean University of China, Yushan Road 5, Qingdao, 266003, China.
J Environ Manage. 2025 Aug;390:126253. doi: 10.1016/j.jenvman.2025.126253. Epub 2025 Jun 21.
Nitrogen pollution in water environments is increasingly severe, and green microbial nitrogen removal has gained significant attention because of its high efficiency, safety, low cost, and eco-friendliness. A strain HHVWA23 with heterotrophic nitrification aerobic denitrification (HNAD) capabilities was isolated from an aquaculture environment and identified as Alcaligenes sp. The removal efficiencies for total-nitrogen (TN), ammonia-nitrogen (NH-N) and nitrite-nitrogen (NO-N) were 99 %, 98 % and 89 %, respectively. The bacterium was encapsulated using bamboo powder, chitosan, and carboxymethyl cellulose through immobilization technology. Two different concentrations of bamboo powder were tested to optimize the carbon source requirements, enhancing the strain's environmental adaptability. The efficiency of nitrogen removal under temperature from 18 to 34 °C, C/N ratio from 2.5 to 20, and salinity from 10 to 50 over 85 %. After 30 days of storage or 3 cycles of reuse of bamboo-powder-microbial-microcapsule (BP-MM) the nitrogen removal rate reached 93 %. Furthermore, the nitrogen removal efficiency in both aquaculture and domestic wastewater was higher than the free HHVWA23, and 2 % BP-MM could completely remove nitrogen from the wastewater. This study successfully developed BP-MM with outstanding performance in colonization, stability, nitrogen removal, and environmental adaptability, thus providing valuable technical support for mitigating nitrogen pollution in aquatic environments.
水环境中的氮污染日益严重，绿色微生物脱氮因其高效、安全、低成本和环保等特点而备受关注。从水产养殖环境中分离出一株具有异养硝化好氧反硝化（HNAD）能力的菌株HHVWA23，并鉴定为产碱菌属。该菌株对总氮（TN）、氨氮（NH-N）和亚硝酸盐氮（NO-N）的去除效率分别为99%、98%和89%。通过固定化技术，利用竹粉、壳聚糖和羧甲基纤维素对该细菌进行包封。测试了两种不同浓度的竹粉以优化碳源需求，提高菌株的环境适应性。在温度为18至34°C、碳氮比为2.5至20、盐度为10至50的条件下，氮去除效率超过85%。竹粉微生物微胶囊（BP-MM）储存30天或重复使用3个循环后，氮去除率达到93%。此外，在水产养殖废水和生活污水中的氮去除效率均高于游离的HHVWA23，2%的BP-MM可完全去除废水中的氮。本研究成功开发出在定殖、稳定性、氮去除和环境适应性方面具有优异性能的BP-MM，为减轻水环境中的氮污染提供了有价值的技术支持。
