Xie Zhuobin, Wang Yun, Jiang Gangqiang, Li Yuwei, Li Wenchang, Liu Yifan, Wu Zhangxiu, Huang Yuanyuan, Tang Shukun
School of Science and Technology, Lingnan Normal University, Zhanjiang 524048, Guangdong, China.
Urumqi Customs Technology Center, Urumqi 830063, Xinjiang, China.
Sheng Wu Gong Cheng Xue Bao. 2025 Jun 25;41(6):2467-2482. doi: 10.13345/j.cjb.240749.
The biological nitrogen removal technology utilizing heterotrophic nitrification-aerobic denitrification (HN-AD) bacteria has shown effectiveness in wastewater treatment. However, the nitrogen removal efficiency of HN-AD bacteria significantly decreases as the salinity increases. To tackle the challenge of treating high-salt and high-nitrogen wastewater, we isolated a moderately halophilic HN-AD strain 5505 from a salt lake in Xinjiang. The strain was identified based on morphological, physiological, and biochemical characteristics and the 16S rRNA gene sequence. Single-factor experiments were carried out with NH-N, NO-N, and NO-N as sole or mixed nitrogen sources to study the nitrifying effect, denitrifying effect, and nitrogen metabolism pathway of the strain. The strain was identified as sp.. It can grow in the presence of 1%-25% (/) NaCl and exhibited efficient nitrogen removal ability in the presence of 3%-8% NaCl. At the optimal NaCl concentration (8%), the strain showed the NH-N, NO-N and NO-N removal rates of 100.0%, 94.11% and 74.43%, respectively. Strain 5505 removed inorganic nitrogen mainly by assimilation, which accounted for over 62.68% of total nitrogen removal. In the presence of mixed nitrogen sources, strain 5505 showed a preference for utilizing ammonia, with a potential HN-AD pathway of NH→NHOH→NO→NO→NO→NO/NO/N. The findings provide efficient salt-tolerant bacterial resources, enhance our understanding of biological nitrogen removal, and contribute to the nitrogen removal efficiency improvement in the treatment of high-salt and high-nitrogen wastewater.
利用异养硝化-好氧反硝化（HN-AD）细菌的生物脱氮技术在废水处理中已显示出有效性。然而，随着盐度的增加，HN-AD细菌的脱氮效率显著降低。为应对处理高盐高氮废水的挑战，我们从新疆的一个盐湖中分离出了一株中度嗜盐的HN-AD菌株5505。基于形态、生理和生化特征以及16S rRNA基因序列对该菌株进行了鉴定。以NH-N、NO-N和NO-N作为单一或混合氮源进行单因素实验，以研究该菌株的硝化作用、反硝化作用和氮代谢途径。该菌株被鉴定为 sp.。它能在1%-25%（/）NaCl存在的情况下生长，并在3%-8%NaCl存在时表现出高效的脱氮能力。在最佳NaCl浓度（8%）下，该菌株对NH-N、NO-N和NO-N的去除率分别为100.0%、94.11%和74.43%。菌株5505主要通过同化作用去除无机氮，同化作用占总氮去除量的62.68%以上。在混合氮源存在的情况下，菌株5505表现出对氨的利用偏好，其潜在的HN-AD途径为NH→NHOH→NO→NO→NO→NO/NO/N。这些发现提供了高效的耐盐细菌资源，增进了我们对生物脱氮的理解，并有助于提高高盐高氮废水处理中的脱氮效率。
