Wang Xue-Yan, Pan Li-Jun, Yang Bing, Liu Yang, Tang Dong-Xin
The First College of Clinical Medicine, Guizhou University of Traditional Chinese Medicine, Guiyang Guizhou, 550005, China.
Department of Medical Affairs, The First Affiliated Hospital of Guizhou, University of Traditional Chinese Medicine, Guiyang Guizhou, 550001, China.
Cancer Cell Int. 2025 Jun 22;25(1):228. doi: 10.1186/s12935-025-03861-4.
To examine the impact of different intestinal microbiota conditions on the absorption of couplet medicines (dried toad skin and radix clematidis) into the bloodstream, and to evaluate the therapeutic effects of drug-containing plasma, produced under different intestinal microbiota conditions, on colorectal cancer HT-29 cells.
In Experiment I, after the pseudo-sterile rat model was established, intragastric administration was performed. Explore the influence of different states of intestinal bacteria on rat organ coefficients, intestine bacteria, plasma metabolites, and so on. In Experiment II, the HT-29 cells of colon cancer were given to each group of drug-containing plasma for intervention to explore the intervention effect of HT-29-cell plasma produced under the influence of different states of intestinal bacteria.
Pseudo-sterile conditions can affect the body weight, organ coefficients, and immune cell ratios of rats, leading to dysbiosis in various segments of their intestinal microbiota. After administering traditional Chinese medicine under pseudo-sterile conditions, the immune cell ratios in rats returned to normal, and the dysbiosis in the intestinal microbiota improved. There were 271 differential metabolites in the plasma between the groups. Cellular experiments indicate that plasma containing drugs obtained under normal gut microbiota conditions can significantly inhibit the proliferation and migration of HT-29 cells (p < 0.01), while the inhibitory effect of plasma containing drugs obtained under dysbiotic gut microbiota conditions is reduced (p < 0.05).
There is a bidirectional regulatory effect between the gut microbiota and the core medicinal pair. On one hand, dysbiosis weakens the efficacy of the medication. Dysbiosis can affect the blood components of the core medicinal pair. Compared to the drug-containing plasma produced under normal gut microbiota conditions, the drug-containing plasma produced under dysbiosis conditions has a reduced inhibitory effect on the proliferation of HT-29 cells. On the other hand, the drug repairs certain functions of the microbiota. After the drug enters the intestine, it exerts a positive regulatory effect on the dysbiosis of the intestinal microbiota in rats, partially improving the dysbiosis caused by antibiotics, restoring the balance of CD3+, CD4+, and CD8 + ratios in rats, and partially restoring the anticancer activity of drug-containing plasma.
not applicable.
研究不同肠道微生物群条件对联用药（干蟾皮和威灵仙）吸收入血的影响，并评估在不同肠道微生物群条件下产生的含药血浆对结直肠癌HT - 29细胞的治疗效果。
实验一，建立伪无菌大鼠模型后进行灌胃给药。探究不同肠道细菌状态对大鼠器官系数、肠道细菌、血浆代谢产物等的影响。实验二，将各组含药血浆给予结肠癌HT - 29细胞进行干预，探究在不同肠道细菌状态影响下产生 的HT - 29细胞血浆的干预效果。
伪无菌条件可影响大鼠体重、器官系数及免疫细胞比例，导致其肠道微生物群各节段生态失调。在伪无菌条件下给予中药后，大鼠免疫细胞比例恢复正常，肠道微生物群失调情况改善。各组间血浆中有271种差异代谢产物。细胞实验表明，正常肠道微生物群条件下获得的含药血浆可显著抑制HT - 29细胞的增殖和迁移（p＜0.01），而在肠道微生物群失调条件下获得的含药血浆的抑制作用减弱（p＜0.05）。
肠道微生物群与核心药对之间存在双向调节作用。一方面，生态失调会削弱药物疗效。生态失调会影响核心药对的血液成分。与正常肠道微生物群条件下产生的含药血浆相比，在生态失调条件下产生的含药血浆对HT - 29细胞增殖的抑制作用减弱。另一方面，药物可修复微生物群的某些功能。药物进入肠道后，对大鼠肠道微生物群失调发挥正向调节作用，部分改善抗生素引起的失调，恢复大鼠CD3 +、CD4 +和CD8 +比例的平衡，并部分恢复含药血浆的抗癌活性。
不适用。
