Xu Wandi, Shi Hongyue, Li Yingdong
College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, China.
Chronobiol Int. 2025 Aug;42(8):1002-1012. doi: 10.1080/07420528.2025.2519308. Epub 2025 Jun 23.
Feeding time is a critical factor influencing the growth, feeding efficiency, and health of animals. This study aimed to investigate the impact of different feeding times on the gut microbiome composition in tadpoles over a 50-d period. Using 16S rRNA sequencing, we analyzed the gut microbiome at four feeding times: 6:00, 12:00, 18:00, and 24:00. Our results revealed that tadpoles fed at 12:00 and 18:00 grew significantly faster than those fed at 6:00 and 24:00. The highest microbial abundance and diversity were observed in the 12:00 feeding group. Additionally, three probiotic bacteria-, , and -were identified as dominant species in this group. These findings suggest that feeding time not only influences the composition and structure of the intestinal microbiota in tadpoles but also affects their growth performance. These results highlight the importance of feeding time in improving tadpole health and farming efficiency.
喂食时间是影响动物生长、喂食效率和健康的关键因素。本研究旨在调查不同喂食时间对蝌蚪肠道微生物群组成在50天期间的影响。我们使用16S rRNA测序技术，分析了四个喂食时间点（6:00、12:00、18:00和24:00）的蝌蚪肠道微生物群。我们的结果显示，在12:00和18:00喂食的蝌蚪比在6:00和24:00喂食的蝌蚪生长明显更快。在12:00喂食组中观察到最高的微生物丰度和多样性。此外，三种益生菌——[此处原文缺失具体菌名]、[此处原文缺失具体菌名]和[此处原文缺失具体菌名]——被确定为该组中的优势菌种。这些发现表明，喂食时间不仅影响蝌蚪肠道微生物群的组成和结构，还会影响它们的生长性能。这些结果凸显了喂食时间在改善蝌蚪健康和养殖效率方面的重要性。
