Ji Haoyu, Shan Baolong, Hu Nan, Zhang Mingchao, Li Yingdong
College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China.
Animals (Basel). 2025 Jul 13;15(14):2066. doi: 10.3390/ani15142066.
, widely distributed and extensively farmed in northeast China, holds significant economic value, particularly for its fallopian tubes, which are used as a traditional Chinese medicinal tonic known as "Oviductus Ranae." As the light spectrum is a cost-effective regulatory factor in aquaculture, understanding its effects on the tadpole stage of is critical for optimizing cultivation practices. This study investigated the effects of five light colors (white, red, yellow, blue, and green) on steroid hormone levels and gut microbiota composition in tadpoles. Steroid hormone levels were measured on days 15, 30, 45, and 60 using high-performance liquid chromatography (HPLC), while gut microbial communities were analyzed through high-throughput 16S rRNA sequencing. Results showed that the testosterone (T) level of frogs in green light (group G) peaked on day 60 (2.62 ± 3.70 ng/g). The estradiol (E2) level in blue light (group B) also peaked on day 60 (2.87 ± 0.71 ng/g). Importantly, sex ratio analysis revealed that the proportion of females was highest under blue light, reaching 61.11%. Meanwhile, the richness and diversity of the gut bacterial community of the tadpoles was highest under yellow light, followed by blue light. These data suggest that hormone levels fluctuated and the composition of the gut flora of changed under different light colors. Our results advance physiological knowledge and support aquaculture practices.
在中国东北广泛分布且大量养殖，具有重要经济价值，尤其是其输卵管，被用作名为“林蛙油”的传统中药滋补品。由于光谱是水产养殖中一种具有成本效益的调控因素，了解其对[物种名称未给出]蝌蚪阶段的影响对于优化养殖实践至关重要。本研究调查了五种光颜色（白色、红色、黄色、蓝色和绿色）对[物种名称未给出]蝌蚪类固醇激素水平和肠道微生物群组成的影响。使用高效液相色谱法（HPLC）在第15、30、45和60天测量类固醇激素水平，同时通过高通量16S rRNA测序分析肠道微生物群落。结果表明，绿光（G组）下青蛙的睾酮（T）水平在第60天达到峰值（2.62±3.70 ng/g）。蓝光（B组）下的雌二醇（E2）水平在第60天也达到峰值（2.87±0.71 ng/g）。重要的是，性别比例分析显示，蓝光下雌性比例最高，达到61.11%。同时，蝌蚪肠道细菌群落的丰富度和多样性在黄光下最高，其次是蓝光。这些数据表明，在不同光颜色下，[物种名称未给出]的激素水平波动，肠道菌群组成发生变化。我们的结果推进了[物种名称未给出]的生理学知识，并支持水产养殖实践。
