Manuilova E S, Lukash L L, Shapiro N I
Genetika. 1985 Aug;21(8):1319-26.
We studied the effect of 12-O-tetradecanoylphorbol-13-acetate (TPA) and saccharin on the frequency of induced mutations of resistance to 6-mercaptopurine and ouabain in Chinese hamster and mouse cells. UV-rays, bovine adenovirus-3 (BAV-3) and 5-bromdeoxyuridine (BrdU) were used as mutagens. In the case of BAV-3 and BrdU, we investigated, apart from the mutagenic effect, the tumor-inducing activity of these mutagens in mice, BrdU proved to have no carcinogenic effect. The data about the influence of TPA on the mutagenic effect of the three different mutagens indicate that TPA increases the frequency of the gene mutations induced by UV-rays and BAV-3. The results of the study of BrdU and TPA combined action revealed the fact that TPA does not increase the mutagenic effect of BrdU. We demonstrated that saccharin also possesses the promoter activity; it increases the mutagenic effect of BAV-3. The results described above lead to the assumption that TPA influence on the mutagenic effect only takes place when carcinogenic mutagens are used.
我们研究了12-O-十四烷酰佛波醇-13-乙酸酯（TPA）和糖精对中国仓鼠及小鼠细胞中诱导产生的对6-巯基嘌呤和哇巴因抗性突变频率的影响。紫外线、牛腺病毒-3（BAV-3）和5-溴脱氧尿苷（BrdU）用作诱变剂。对于BAV-3和BrdU，除了诱变作用外，我们还研究了这些诱变剂在小鼠中的致瘤活性，结果表明BrdU没有致癌作用。关于TPA对三种不同诱变剂诱变作用影响的数据表明，TPA增加了紫外线和BAV-3诱导的基因突变频率。对BrdU和TPA联合作用的研究结果表明，TPA不会增加BrdU的诱变作用。我们证明糖精也具有启动子活性；它增加了BAV-3的诱变作用。上述结果导致这样一种假设，即只有当使用致癌诱变剂时，TPA才会对诱变作用产生影响。
