Fang Zhijian, Zhao Hong, Cheng Yongpeng, Yu Long
Department of Hepatobiliary Surgery, Liupanshui People's Hospital, Liupanshui, Guizhou Province, 553000, People's Republic of China.
J Inflamm Res. 2025 Jun 17;18:7921-7931. doi: 10.2147/JIR.S527630. eCollection 2025.
This study investigated the association of serum Trefoil Factor 2 (TFF2) and microRNA-186-5p (miR-186-5p) levels with the severity and prognosis of acute pancreatitis (AP).
A retrospective analysis was conducted on 380 AP patients, classified into mild to moderately severe (mild acute pancreatitis (MAP) and moderately severe acute pancreatitis (MSAP), n=205) and severe (SAP, n=175) groups. Serum TFF2 levels were measured by enzyme-linked immunosorbent assay (ELISA), and miR-186-5p expression was quantified via reverse transcription quantitative polymerase chain reaction (RT-qPCR). Correlations with inflammatory markers (high-sensitivity C-reactive protein (hs-CRP), interleukin-18 (IL-18), and tumor necrosis factor-α (TNF-α)) and Acute Physiology and Chronic Health Evaluation II (APACHE II) scores were assessed using Pearson analysis. Diagnostic performance was evaluated using receiver operating characteristic (ROC) curves, and logistic regression was used to identify risk factors for SAP.
Compared with the MAP&MSAP group, the SAP group showed significantly elevated TFF2, hs-CRP, IL-18, TNF-α levels, and APACHE II scores, while miR-186-5p levels were significantly reduced (P < 0.05). TFF2 levels were positively correlated with inflammatory markers and APACHE II scores (r = 0.427-0.546), whereas miR-186-5p levels showed negative correlations (r = -0.431 to -0.570; all P < 0.05). TFF2 and miR-186-5p levels were inversely correlated (r = -0.483, P < 0.05). ROC analysis yielded AUCs of 0.804 for TFF2, 0.832 for miR-186-5p, and 0.895 for their combination in predicting SAP. Logistic regression identified TFF2 as an independent risk factor and miR-186-5p as a protective factor for SAP (P < 0.05).
Elevated serum TFF2 level and reduced miR-186-5p level were found to be associated with increased AP severity. These biomarkers may serve as useful indicators for assessing disease severity and prognosis in AP.
本研究探讨血清三叶因子2（TFF2）和微小RNA - 186 - 5p（miR - 186 - 5p）水平与急性胰腺炎（AP）严重程度及预后的关系。
对380例AP患者进行回顾性分析，分为轻度至中度严重组（轻度急性胰腺炎（MAP）和中度严重急性胰腺炎（MSAP），n = 205）和严重组（SAP，n = 175）。采用酶联免疫吸附测定（ELISA）法检测血清TFF2水平，通过逆转录定量聚合酶链反应（RT - qPCR）对miR - 186 - 5p表达进行定量分析。采用Pearson分析评估与炎症标志物（高敏C反应蛋白（hs - CRP）、白细胞介素 - 18（IL - 18）和肿瘤坏死因子 - α（TNF - α））及急性生理与慢性健康状况评分系统II（APACHE II）评分的相关性。使用受试者工作特征（ROC）曲线评估诊断性能，并采用逻辑回归分析确定SAP的危险因素。
与MAP&MSAP组相比，SAP组的TFF2、hs - CRP、IL - 18、TNF - α水平及APACHE II评分显著升高，而miR - 186 - 5p水平显著降低（P < 0.05）。TFF2水平与炎症标志物及APACHE II评分呈正相关（r = 0.427 - 0.546），而miR - 186 - 5p水平呈负相关（r = - 0.431至 - 0.570；均P < 0.05）。TFF2与miR - 186 - 5p水平呈负相关（r = - 0.483，P < 0.05）。ROC分析显示，TFF2预测SAP的曲线下面积（AUC）为0.804，miR - 186 - 5p为0.832，两者联合为0.895。逻辑回归分析确定TFF2为SAP的独立危险因素，miR - 186 - 5p为保护因素（P < 0.05）。
血清TFF2水平升高和miR - 186 - 5p水平降低与AP严重程度增加有关。这些生物标志物可作为评估AP疾病严重程度和预后的有用指标。
