Biketova Alona Yu, Svetasheva Tatyana Yu, Taylor Andy F S, Simonini Giampaolo, Gelardi Matteo, Morozova Olga V, Polemis Elias, Muñoz José A, Albert László, Saitta Salvatore, Wasser Solomon P, Nevo Eviatar, Zervakis Georgios I, Vizzini Alfredo, Dima Bálint
Jodrell Laboratory, Royal Botanic Gardens, Kew, Richmond TW9 3DS, UK Jodrell Laboratory, Royal Botanic Gardens, Kew Richmond United Kingdom.
Mycological Society of Israel, P.O. Box 164, Pardesiya 42815, Israel University of Haifa Haifa Israel.
IMA Fungus. 2025 Jun 12;16:e144731. doi: 10.3897/imafungus.16.144731. eCollection 2025.
(the former species complex) is one of the most taxonomically critical and difficult genera for species identification in the family . Here, we provide a detailed morphological and molecular re-assessment of European and Levantine species of . A new species, , is described from Israel. It is sister to and associated with the evergreen oak and potentially also with . Based on the sequence retrieved from INSDC, this species is also found in Lebanon. Accurate morphological descriptions, comprehensive sampling, type studies, biogeography, macro- and microphotographs and a historical overview on the nomenclatural issues surrounding , , , and are given. An epitype collection is designated for . A key is provided for identification of the European and Levantine taxa. In addition, we propose a novel taxonomic combination , which is conspecific with Boletusrubellusvar.flammeus, based on the DNA barcoding and phylogenetic analysis of type material. is also shown to be conspecific with . A multilocus phylogenetic analysis of four markers (ITS, LSU, , and ) reveals that is a sister genus to . Using the Genealogical Concordance Phylogenetic Species Recognition method, at least 19 phylogenetic species and eight putative phylogenetic species of the genus can be delimited. Based on multilocus analysis, it contains from 24 to 25 species-level clades worldwide, 17 out of which represent known species, one newly described and potentially six to seven undescribed species. Tandem repeat insertions within the ITS region (both in ITS1 and ITS2) are reported for the first time, not only in the genus , but in the entire subfamily . Their identification and characterisation were based on Tandem Repeat Finder analysis and visual assessment of the ITS alignment.
(前一个物种复合体)是该科中物种鉴定在分类学上最关键且最困难的属之一。在此,我们对欧洲和黎凡特地区的该属物种进行了详细的形态学和分子重新评估。描述了一个来自以色列的新物种。它是[具体物种A]的姐妹种,与常绿栎[具体栎树种类]相关,并且可能也与[另一相关物种]有关。根据从国际核苷酸序列数据库协作中心(INSDC)检索到的序列,该物种在黎巴嫩也有发现。给出了准确的形态学描述、全面的采样、模式研究、生物地理学、宏观和微观照片以及围绕[相关物种A]、[相关物种B]、[相关物种C]和[相关物种D]的命名问题的历史概述。为[相关物种A]指定了一个后选模式标本。提供了一个用于鉴定欧洲和黎凡特分类群的检索表。此外,基于模式材料的DNA条形码和系统发育分析,我们提出了一个新的分类组合[新组合名称],它与红绒牛肝菌火焰变种(Boletusrubellusvar.flammeus)同种。[具体物种B]也被证明与[另一物种]同种。对四个标记(ITS、LSU、[具体标记C]和[具体标记D])的多位点系统发育分析表明,[具体属名]是[另一属名]的姐妹属。使用系统发育物种识别的谱系一致性方法,可以界定该属至少19个系统发育物种和8个假定的系统发育物种。基于多位点分析,它在全球包含24至25个物种水平的分支,其中17个代表已知物种,1个新描述物种以及可能6至7个未描述物种。首次报道了ITS区域(包括ITS1和ITS2)内的串联重复插入,不仅在该属中,而且在整个亚科中。它们的鉴定和特征描述基于串联重复序列查找器分析和ITS比对的视觉评估。