Isidan Hakan, Turan Turhan
Department of Virology, Faculty of Veterinary Medicine, Sivas Cumhuriyet University, Sivas, Turkey.
Vet Med (Praha). 2021 Oct 31;66(10):423-430. doi: 10.17221/130/2020-VETMED. eCollection 2021 Oct.
A total of 150 rectal swab samples were collected from diarrheic dogs from the Sivas Municipal Animal Shelter, Turkey in April 2018. While 127 faecal samples were gathered from adults, 23 samples were collected from puppies. Canine parvovirus type 2 (Carnivore protoparvovirus 1), Canine minute virus (Carnivore bocaparvovirus 1) and Canine bocavirus (Carnivore bocaparvovirus 2) were examined by PCR with three sets of novel primers. Some of the amplicons were subjected to molecular sequencing and molecular analysis. Three faecal (2.36%) samples were found to be positive for Carnivore protoparvovirus 1, five faecal samples (3.94%) were found to be positive for Carnivore bocaparvovirus 1 and three samples (2.36%) were found to be positive for Carnivore bocaparvovirus 2 among 127 adult dogs. On the other hand, 14/23 faecal samples (60.87%) were found to be positive for Carnivore protoparvovirus 1, 8/23 faecal samples (34.78%) were found to be positive for Carnivore bocaparvovirus 1, and 6/23 faecal samples (26.09%) were found to be positive for Carnivore bocaparvovirus 2 from the puppies. Moreover, we detected two distinct clades of Carnivore bocaparvovirus 1 and 2 according to the molecular analysis. To the best of our knowledge, this is the first report for the direct detection of Carnivore bocaparvovirus 1 and 2 in Turkey.
2018年4月，从土耳其锡瓦斯市动物收容所腹泻犬只中总共采集了150份直肠拭子样本。其中，127份粪便样本采自成年犬，23份样本采自幼犬。使用三组新型引物通过聚合酶链反应（PCR）检测了2型犬细小病毒（肉食动物原细小病毒1型）、犬微小病毒（肉食动物博卡细小病毒1型）和犬博卡病毒（肉食动物博卡细小病毒2型）。部分扩增子进行了分子测序和分子分析。在127只成年犬中，发现3份粪便样本（2.36%）对肉食动物原细小病毒1型呈阳性，5份粪便样本（3.94%）对肉食动物博卡细小病毒1型呈阳性，3份样本（2.36%）对肉食动物博卡细小病毒2型呈阳性。另一方面，在幼犬中，发现14/23份粪便样本（60.87%）对肉食动物原细小病毒1型呈阳性，8/23份粪便样本（34.78%）对肉食动物博卡细小病毒1型呈阳性，6/23份粪便样本（26.09%）对肉食动物博卡细小病毒2型呈阳性。此外，根据分子分析，我们检测到肉食动物博卡细小病毒1型和2型的两个不同进化枝。据我们所知，这是土耳其首次直接检测到肉食动物博卡细小病毒1型和2型的报告。
