Lee Ho Jae, Jeong Chang Hee, Kim Tae-Woon, Seo Sang Wan, Jeong Do-Won, Hong Sung Wook
Technology Innovation Research Division, World Institute of Kimchi, Gwangju, 61755, Republic of Korea.
Department of Biomedical Laboratory Science, Honam University, Gwangju, 62399, Republic of Korea.
Braz J Microbiol. 2025 Jun 23. doi: 10.1007/s42770-025-01703-z.
Recent years have seen an increase in the incidence of acne in adults and teenagers resulting in active research in acne treatment. In the present study, we aimed to improve the anti-acne effects of Hwangryeonhaedok-tang (HHT) through fermentation using a kimchi-derived lactic acid bacterium. Lactiplantibacillus plantarum WiKim0111 (Lpb. plantarum WiKim0111) was selected to ferment HHT due to its strong antioxidant activity and its ability to effectively survive and grow in the HHT medium. The antimicrobial activity of HHT and fermented HHT (FHHT) against Cutibacterium acnes (C. acnes) was determined using an agar well diffusion assay. FHHT exhibited stronger antimicrobial, radical scavenging, and superoxide dismutase activity compared to HHT. RAW 264.7 cells pretreated HHT or FHHT showed significantly lower production of nitric oxide, interleukin (IL) -1β, and IL-6 than lipopolysaccharide -treated cells, with FHHT demonstrating higher efficiency than HHT. Overall, HHT fermented by Lpb. plantarum WiKim0111 displayed enhanced antioxidant and anti-inflammatory properties, as well as greater antibacterial activity against C. acnes compared to unfermented HHT. These findings suggest that FHHT may help mitigate acne symptoms by reducing oxidative stress, modulating inflammation, and directly inhibiting the growth of C. acnes. Further in vitro studies focusing on sebum-producing skin cells, as well as clinical trials evaluating the topical or oral application of FHHT, would be valuable to confirm its efficacy and safety in acne treatment.
近年来，成人和青少年痤疮的发病率有所上升，这导致了对痤疮治疗的积极研究。在本研究中，我们旨在通过使用泡菜衍生的乳酸菌发酵来提高黄连解毒汤（HHT）的抗痤疮效果。由于植物乳杆菌WiKim0111（Lpb. plantarum WiKim0111）具有强大的抗氧化活性以及在HHT培养基中有效存活和生长的能力，因此选择它来发酵HHT。使用琼脂孔扩散法测定HHT和发酵后的HHT（FHHT）对痤疮丙酸杆菌（C. acnes）的抗菌活性。与HHT相比，FHHT表现出更强的抗菌、自由基清除和超氧化物歧化酶活性。用HHT或FHHT预处理的RAW 264.7细胞显示，与用脂多糖处理的细胞相比，一氧化氮、白细胞介素（IL）-1β和IL-6的产生显著降低，且FHHT的效率高于HHT。总体而言，与未发酵的HHT相比，由植物乳杆菌WiKim0111发酵的HHT具有增强的抗氧化和抗炎特性，以及对痤疮丙酸杆菌更强的抗菌活性。这些发现表明，FHHT可能通过减少氧化应激、调节炎症和直接抑制痤疮丙酸杆菌的生长来帮助减轻痤疮症状。进一步针对产生皮脂的皮肤细胞的体外研究，以及评估FHHT局部或口服应用的临床试验，对于确认其在痤疮治疗中的疗效和安全性将是有价值的。
