Sharma Anshula, Gupta Gaganjot, Ahmad Tawseef, Kaur Baljinder, Hakeem Khalid Rehman
Department of Biotechnology, Punjabi University, Patiala 147002, India.
Department of Biotechnology, Punjabi University, Patiala 147002, India.
J Microbiol Methods. 2020 Mar;170:105862. doi: 10.1016/j.mimet.2020.105862. Epub 2020 Feb 5.
Metabolic engineering combines approaches of synthetic and systems biology for tailoring the existing and creating novel biosynthetic metabolic pathways in the desired industrial microorganisms for production of biofuels, bio-materials and environmental applications. Lactic acid bacteria (LAB) are gaining attention worldwide due to their extensive utilization in food, fermentation and pharmaceutical industries owing to their GRAS status. Well-elucidated genetics and regulatory control of central metabolism make them potential candidates for the production of industrially valuable metabolites. With the recent advancements in metabolic engineering strategies, genetic manipulation and tailoring of cellular metabolism is being successfully carried out in various LAB strains as they are providing highly efficient and industrially competitive robust expression systems. Thus, this review presents a concise overview of metabolic engineering strategies available for the comprehensive tailoring of lactic acid bacterial strains for large-scale production of industrially important metabolites.
代谢工程结合了合成生物学和系统生物学方法，用于在所需的工业微生物中定制现有的生物合成代谢途径并创建新的途径，以生产生物燃料、生物材料和用于环境应用。乳酸菌（LAB）因其公认安全（GRAS）的地位而在食品、发酵和制药行业中广泛应用，从而在全球范围内受到关注。对中心代谢的充分阐明的遗传学和调控控制使其成为生产具有工业价值的代谢物的潜在候选者。随着代谢工程策略的最新进展，由于各种乳酸菌菌株提供了高效且具有工业竞争力的强大表达系统，因此正在成功地对其进行细胞代谢的基因操作和定制。因此，本综述简要概述了可用于全面定制乳酸菌菌株以大规模生产具有工业重要性的代谢物的代谢工程策略。
