Cameron D J
Immunol Lett. 1985;11(1):39-44. doi: 10.1016/0165-2478(85)90140-3.
In this study, we have examined the role cell surface carbohydrates play in the activation of macrophages to kill tumor cells as well as the effect removal of tumor cell surface fucose has on the susceptibility of the treated target cells to the cytotoxic macrophages. After incubation of human monocyte-derived macrophages with alpha-L-fucosidase, a glycosidase which splits terminal alpha-L-fucose from oligosaccharides, the macrophages were no longer able to respond to lipopolysaccharide (LPS). These experiments strongly suggest that alpha-L-fucose probably comprises an essential part of the macrophage membrane receptor for LPS. In another series of experiments tumor cells were treated with alpha-L-fucosidase prior to co-cultivation with macrophages to determine whether the presence of alpha-L-fucose on the tumor cell surface had any effect on macrophage mediated cytotoxicity. It was found that the MA-160 sensitive tumor target, after alpha-L-fucosidase treatment, became resistant to the effects of the cyto-toxic macrophages whereas the effect of the alpha-L-fucosidase treatment was blocked by addition of alpha-L-fucose to the incubation mixture.
在本研究中，我们研究了细胞表面碳水化合物在激活巨噬细胞以杀伤肿瘤细胞中所起的作用，以及去除肿瘤细胞表面岩藻糖对经处理的靶细胞对细胞毒性巨噬细胞的敏感性的影响。在用α-L-岩藻糖苷酶（一种从寡糖中裂解末端α-L-岩藻糖的糖苷酶）孵育人单核细胞衍生的巨噬细胞后，巨噬细胞不再能够对脂多糖（LPS）作出反应。这些实验强烈表明，α-L-岩藻糖可能是LPS巨噬细胞膜受体的重要组成部分。在另一系列实验中，在与巨噬细胞共培养之前，先用α-L-岩藻糖苷酶处理肿瘤细胞，以确定肿瘤细胞表面α-L-岩藻糖的存在是否对巨噬细胞介导的细胞毒性有任何影响。结果发现，经α-L-岩藻糖苷酶处理后，对MA-160敏感的肿瘤靶细胞对细胞毒性巨噬细胞的作用产生了抗性，而在孵育混合物中添加α-L-岩藻糖可阻断α-L-岩藻糖苷酶处理的效果。
