Prabhakar Patole Reshma, Reddy Jeevan Prasad, Murthy P S Keshava, Babu C S Vivek
Food Protectants & Infestation Control (FPIC) Department, CSIR-Central Food Technological Research Institute, Mysuru, India; Academy of Scientific and Innovative Research, (AcSIR), India.
Polymer Materials Lab, Food Packaging Technology Department, CSIR-Central Food Technological Research Institute, Mysuru, India; Academy of Scientific and Innovative Research, (AcSIR), India.
Int J Biol Macromol. 2025 Aug;319(Pt 2):145447. doi: 10.1016/j.ijbiomac.2025.145447. Epub 2025 Jun 21.
Current study, highlights development of composite mixture of polylactic acid (PLA) and polybutylene adipate terephthalate (PBAT) incorporated with phytochemicals (thymol, α-pinene, eucalyptol, estragole) as insecticidal biopolymers. Further, these developed films were analysed with gas chromatography-mass spectrometry (GC-MS) coupled with solid-phase microextraction (SPME) and results showed the presence of thymol, α-pinene, eucalyptol, estragole in the composite films (PLA-PBAT-T 0.5 %, PLA-PBAT- A 4.5 %, PLA-PBAT-E 4.5 %). The synthesized films showed significant mortality (100 %) towards Sitophilus oryzae within 72 h whereas Oryzophilus surinamensis exhibited complete mortality within 48 h in presence of grain (pearl millet) conditions. Seed germination study showed seed viability (100 %) in the tested grains which was equivalent to fresh grains. Mechanical property (tensile strength) was reduced (lowest in PLA-PBAT-AT 5 % - 10.44 MPa), but the water vapour transmission rate (WVTR) was found to be significant in PLA-PBAT-T 0.5 % blend (13.63 g/m.hr). The XRD analysis showed increase in the crystallinity index of PLA-PBAT-T 0.5 % (15.68 %), PLA-PBAT- AT 5 % (14.75 %) and PLA-PBAT- ET 5 % (13.77 %) films. Fourier transform infrared spectroscopy and scanning electron microscopy analysis of the biopolymer films showed complete homogenization of the phytochemicals and weight loss (maximum 4.9 %). Overall, phytochemical amended biopolymer films displayed significant insecticidal activity in prevention of storage pests of pearl millet.
当前的研究重点是开发一种聚乳酸（PLA）和聚己二酸丁二醇酯对苯二甲酸酯（PBAT）的复合混合物，并掺入植物化学物质（百里香酚、α-蒎烯、桉叶油素、草蒿脑）作为杀虫生物聚合物。此外，使用气相色谱-质谱联用仪（GC-MS）结合固相微萃取（SPME）对这些制成的薄膜进行了分析，结果表明复合薄膜（PLA-PBAT-T 0.5%、PLA-PBAT-A 4.5%、PLA-PBAT-E 4.5%）中存在百里香酚、α-蒎烯、桉叶油素、草蒿脑。合成薄膜在72小时内对米象显示出显著的死亡率（100%），而在有谷物（珍珠粟）的条件下，苏里南锯谷盗在48小时内表现出完全死亡。种子发芽研究表明，受试谷物中的种子活力为（100%），与新鲜谷物相当。机械性能（拉伸强度）有所降低（在PLA-PBAT-AT 5%时最低 - 10.44兆帕），但在PLA-PBAT-T 0.5%共混物中发现水蒸气透过率（WVTR）显著（13.63克/平方米·小时）。X射线衍射分析表明，PLA-PBAT-T 0.5%（15.68%）、PLA-PBAT-AT 5%（14.75%）和PLA-PBAT-ET 5%（13.77%）薄膜的结晶度指数有所增加。对生物聚合物薄膜的傅里叶变换红外光谱和扫描电子显微镜分析表明，植物化学物质完全均匀化且有重量损失（最大4.9%）。总体而言，植物化学物质改良的生物聚合物薄膜在预防珍珠粟储存害虫方面表现出显著的杀虫活性。
