Parasitol Res. 2013 Dec;112(12):4137-50. doi: 10.1007/s00436-013-3605-y.
Phyllodistomum inecoli n. sp. is described from the twospot livebearer, Heterandria bimaculata (Teleostei: Poeciliidae), collected in the Río La Antigua basin, Veracruz, Mexico. The new species is described and characterised by using a combination of morphology, scanning electron microscopy, and sequences of nuclear and mitochondrial genes. Diagnostic characters of the new species of Phyllodistomum include a genital pore opening at the level of the caecal bifurcation; oval vitellarium, situated just posterior to the ventral sucker and not extended laterally and anterior extracaecal uterine loops variable in extension (reaching the anterior, median or posterior margin of the ventral sucker). P. inecoli n. sp. most closely resembles P. brevicecum, a species described as a parasite of the central mudminnow, Umbra limi, in other parts of North America; however, the genital pore in P. brevicecum is situated between the caecal bifurcation and the ventral sucker, the ovary is larger, the vitellarium is lobed and extended laterally and the anterior portion of the uterus extends to the posterior margin of the ventral sucker. Comparison of about 1,500–2,200 nucleotides of cox1 and 28S rDNA and ITS1 strongly supports the status of P. inecoli as a new species. Bayesian inference analysis of combined datasets of 28S rDNA and cox1 sequences showed that P. inecoli n. sp. and the other species found in freshwater fishes of Mexico, including the species complex of P. lacustri, are not sister species. Phylogenetic analysis based on 28S rDNA sequences of several gorgoderid taxa revealed the close relationship of P. inecoli n. sp. with several species of Phyllodistomum, Gorgodera and Gorgoderina with cystocercous cercariae developing in sphaeriid bivalves. Dot-plot analysis of ITS1 sequences of P. inecoli n. sp. revealed the presence of eight repetitive elements with different length, which together represent almost half the length of ITS1.
本文描述了一种来自双斑丽鱼（Teleostei: Poeciliidae）的新的菲勒氏吸虫属物种，双斑丽鱼采集于墨西哥韦拉克鲁斯的拉安提瓜流域。该新种是通过形态学、扫描电子显微镜和核基因和线粒体基因序列的组合来描述和特征化的。该新种菲勒氏吸虫的鉴别特征包括生殖孔开口位于盲肠分叉处；卵黄腺呈椭圆形，位于腹吸盘后面，不向侧面延伸，前额外的子宫环在延伸程度上变化（到达腹吸盘的前、中或后缘）。P. inecoli n. sp. 与 P. brevicecum 最为相似，后者是一种描述为北美其他地区中央泥鱼（Umbra limi）寄生虫的物种；然而，P. brevicecum 的生殖孔位于盲肠分叉和腹吸盘之间，卵巢较大，卵黄腺呈叶状并向侧面延伸，子宫的前部延伸至腹吸盘的后缘。cox1 和 28S rDNA 以及 ITS1 约 1500-2200 个核苷酸的比较强烈支持 P. inecoli 作为一个新物种的地位。28S rDNA 和 cox1 序列联合数据集的贝叶斯推断分析表明，P. inecoli n. sp. 和在墨西哥淡水鱼类中发现的其他物种，包括 P. lacustri 物种复合体，不是姐妹种。基于几个 gorgoderid 分类群的 28S rDNA 序列的系统发育分析表明，P. inecoli n. sp. 与几种菲勒氏吸虫、Gorgodera 和 Gorgoderina 密切相关，这些物种的囊尾蚴在 Sphae riid 双壳类中发育。P. inecoli n. sp. 的 ITS1 序列的点图分析显示存在 8 个具有不同长度的重复元件，它们共同代表了 ITS1 长度的近一半。
