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一株新希瓦氏菌的异养硝化和好氧反硝化作用。

Heterotrophic nitrification and aerobic denitrification by a novel Halomonas campisalis.

机构信息

National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Zhongguancun Bei-er-tiao 1, Haidian District, P.O. Box 353, Beijing, 100190, People's Republic of China.

出版信息

Biotechnol Lett. 2013 Dec;35(12):2045-9. doi: 10.1007/s10529-013-1294-3. Epub 2013 Aug 2.

Abstract

A novel halophilic strain that could carry out heterotrophic nitrification and aerobic denitrification was isolated and named as Halomonas campisalis ha3. It removed inorganic nitrogen compounds (e.g. NO3 (-), NO2 (-) and NH4 (+)) simultaneously, and grew well in the medium containing up to 20 % (w/v) NaCl. PCR revealed four genes in the genome of ha3 related to aerobic denitrification: napA, nirS, norB and nosZ. The optimal conditions for aerobic denitrification were pH 9.0, at 37 °C, with 4 % (w/v) NaCl and sodium succinate as carbon source. The nitrogen removal rate was 87.5 mg NO3 (-)-N l(-1 )h(-1). Therefore, this strain is a potential aerobic denitrifier for the treatment of saline wastewater.

摘要

一株能够进行异养硝化和好氧反硝化的嗜盐新菌株被分离出来,并命名为盐单胞菌 ha3。它能同时去除无机氮化合物(如 NO3(-)、NO2(-) 和 NH4(+)),并能在含高达 20%(w/v)NaCl 的培养基中良好生长。PCR 显示 ha3 基因组中与好氧反硝化相关的有四个基因:napA、nirS、norB 和 nosZ。好氧反硝化的最佳条件为 pH9.0、37°C、4%(w/v)NaCl 和琥珀酸钠作为碳源。氮去除率为 87.5 mg NO3(-)-N l(-1) h(-1)。因此,该菌株是处理含盐废水的潜在好氧反硝化菌。

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