Brunner G, Tegtmeier F, Lösgen H
Int J Artif Organs. 1985 Jul;8(4):225-30.
In order to find optimal long term cofactor supply for continuous enzymatic detoxification processes, different resins of varying surface, dipole moment, pore size, and chemical structure were investigated for their adsorptive capacity as well as their desorption behaviour towards various nucleotide based coenzymes. UDPGA, NADPGH, NADH, and SAM were gently shaken with agarose coated resins XAD-12, XAD-8, XAD-7, XAD-4, XAD-2, Dowex 1 X 2 (50-100; 200-400), Dowex 1 X 4 (20-50; 200-400), Dowex 2 X 8, and charcoal until all nucleotide was adsorbed or a saturation of the resins was achieved. High adsorption capacity was not always found to correlate with a steady release of cofactor in desorption experiments. Under this premise the optimal resin-cofactor combinations for long term cofactor supply were found to be XAD-12 for UDPGA, Dowex 2 X 8 for NADPH, Dowex 1 X 4 (20-50) for NADH, and XAD-7 for SAM.
为了找到连续酶解毒过程的最佳长期辅因子供应，研究了具有不同表面、偶极矩、孔径和化学结构的不同树脂对各种基于核苷酸的辅酶的吸附能力及其解吸行为。将尿苷二磷酸葡萄糖醛酸（UDPGA）、还原型辅酶II磷酸酯（NADPGH）、还原型辅酶I（NADH）和S-腺苷甲硫氨酸（SAM）与琼脂糖包被的树脂XAD-12、XAD-8、XAD-7、XAD-4、XAD-2、强碱性阴离子交换树脂Dowex 1 X 2（50 - 100；200 - 400）、Dowex 1 X 4（20 - 50；200 - 400）、Dowex 2 X 8和活性炭轻轻振荡，直至所有核苷酸被吸附或树脂达到饱和。在解吸实验中，高吸附能力并不总是与辅因子的稳定释放相关。在此前提下，发现用于长期辅因子供应的最佳树脂 - 辅因子组合为：UDPGA用XAD-12，NADPH用Dowex 2 X 8，NADH用Dowex 1 X 4（20 - 50），SAM用XAD-7。
